4.7 Article

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Journal

NATURE PROTOCOLS
Volume 18, Issue 6, Pages 1893-1929

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41596-023-00814-x

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Induced pluripotent stem cell-derived brain organoids, known as OVB organoids, can deconstruct the developmental complexities of the human brain and help study interorgan interactions between optic vesicles and the brain. This protocol enables the generation of functional integrated bilateral optic vesicles and can be used to model early eye patterning defects.
Induced pluripotent stem cell-derived brain organoids enable the developmental complexities of the human brain to be deconstructed. During embryogenesis, optic vesicles (OVs), the eye primordium attached to the forebrain, develop from diencephalon. However, most 3D culturing methods generate either brain or retinal organoids individually. Here we describe a protocol to generate organoids with both forebrain entities, which we call OV-containing brain organoids (OVB organoids). In this protocol, we first induce neural differentiation (days 0-5) and collect neurospheres, which we culture in a neurosphere medium to initiate their patterning and further self-assembly (days 5-10). Then, upon transfer to spinner flasks containing OVB medium (days 10-30), neurospheres develop into forebrain organoids with one or two pigmented dots restricted to one pole, displaying forebrain entities of ventral and dorsal cortical progenitors and preoptic areas. Further long-term culture results in photosensitive OVB organoids constituting complementary cell types of OVs, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections and electrically active neuronal networks. OVB organoids provide a system to help dissect interorgan interactions between the OVs as sensory organs and the brain as a processing unit, and can help model early eye patterning defects, including congenital retinal dystrophy. To conduct the protocol, experience in sterile cell culture and maintenance of human induced pluripotent stem cells is essential; theoretical knowledge of brain development is advantageous. Furthermore, specialized expertise in 3D organoid culture and imaging for the analysis is needed. This protocol enables the generation of human forebrain organoids from induced pluripotent stem cells with the intrinsic ability to assemble functionally integrated bilateral optic vesicles.

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