Local translation in microglial processes

Review Biochemistry & Molecular Biology

De-centralizing the Central Dogma: mRNA translation in space and time

Ashley M. Bourke et al.

Summary: As our understanding of cellular processes deepens, we have recognized the localization and translation of mRNAs as important operations within cells. This review examines the features of localized mRNAs, their transport and localization mechanisms, and the regulation of this process. Additionally, it evaluates our current understanding of ribosomes and translation factors.

MOLECULAR CELL (2023)

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Article Neurosciences

Local translation in microglial processes is required for efficient phagocytosis

Michael J. Vasek et al.

Summary: Vasek et al. demonstrate that distal processes of microglia locally translate specific mRNAs related to immunity and phagocytosis. They show that local protein synthesis is necessary for microglial process-initiated phagocytosis. Neurons, astrocytes and oligodendrocytes also regulate protein translation within distal processes. Using a live slice preparation, they further show that acute translation blockade impairs the formation of peripheral microglial process (PeMP) phagocytic cups, lysosomal protein localization, and phagocytosis. These data suggest the importance of regulated local translation in PeMPs for dynamic microglial functions.

NATURE NEUROSCIENCE (2023)

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Article Biochemistry & Molecular Biology

Far from home: the role of glial mRNA localization in synaptic plasticity

Dalia S. Gala et al.

RNA (2022)

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Article Neurosciences

Microglia states and nomenclature: A field at its crossroads

Rosa C. Paolicelli et al.

NEURON (2022)

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Article Biology

Subcellular RNA-seq for the Analysis of the Dendritic and Somatic Transcriptomes of Single Neurons

Julio D. Perez et al.

Summary: In this study, a subcellular single cell RNA sequencing protocol is presented, which allows for molecular quantification from the soma and dendrites of single neurons and can be scaled up for the characterization of several hundreds to thousands of neurons. The protocol provides detailed instructions for both wet lab and bioinformatic steps, as well as insights into controls, data analysis, interpretations, and ways to achieve robust and reproducible results.

BIO-PROTOCOL (2022)

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Review Cell Biology

RNA Localization and Local Translation in Glia in Neurological and Neurodegenerative Diseases: Lessons from Neurons

Maite Blanco-Urrejola et al.

Summary: Cell polarity is crucial for distinct structural and functional domains in cells, with local protein synthesis playing a key role in regulating protein homeostasis. Neurons and glia rely on accurate subcellular protein distribution for their polarized functions.

CELLS (2021)

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Editorial Material Cell Biology