Three new lanostane triterpenoids and two new amides from Alternaria sp. with NLRP3 inflammasome inhibitory activity

Three new lanostane triterpenoids (1-3) along with two new amides fatty compounds (4-5) were isolated from the ethyl acetate extract of a culture of the endophytic fungus Alternaria sp. gx-2. Their structures were identified by 1D and 2D NMR spectral data and HRESIMS. Compounds 1-12 were evaluated for their anti-inflammatory and tyrosinase inhibition activities. The isolated compounds did not show inhibitory activities at a concentration of 100 mu M against tyrosinase, while under the concentration of 10 mu M, the release of lactate dehydrogenase (LDH) inhibition rate of compound 1 was 54.45%, indicating that compound 1 had moderate anti-inflammatory activity on the activation of NLRP3 inflammasome.

Automated summary

Three new lanostane triterpenoids (1-3) and two new amide fatty compounds (4-5) were isolated from the ethyl acetate extract of the endophytic fungus Alternaria sp. gx-2. The structures of these compounds were identified through NMR and HRESIMS analysis. Compound 1 showed moderate anti-inflammatory activity on the activation of NLRP3 inflammasome with a lactate dehydrogenase (LDH) inhibition rate of 54.45% at a concentration of 10 μM.