4.6 Article

Antimicrobial Diterpenes from Rough Goldenrod (Solidago rugosa Mill.)

Journal

MOLECULES
Volume 28, Issue 9, Pages -

Publisher

MDPI
DOI: 10.3390/molecules28093790

Keywords

high-performance thin-layer chromatography-effect-directed analysis; bioassay-guided isolation; antibacterial effect; antifungal effect; (-)-hardwickiic acid; (-)-abietic acid

Ask authors/readers for more resources

Solidago rugosa, a native goldenrod species in North America, has become a sporadic alien plant in Europe. Two antimicrobial components, (-)-hardwickiic acid and (-)-abietic acid, were isolated from the root and leaf ethanol extracts of the plant. Both compounds demonstrated antibacterial activity, especially against Gram-positive bacterial strains. Compound 1 also showed significant antifungal activity against Bipolaris sorokiniana.
Solidago rugosa is one of the goldenrod species native to North America but has sporadically naturalized as an alien plant in Europe. The investigation of the root and leaf ethanol extracts of the plant using a bioassay-guided process with an anti-Bacillus assay resulted in the isolation of two antimicrobial components. Structure elucidation was performed based on high-resolution tandem mass spectrometric and one- and two-dimensional NMR spectroscopic analyses that revealed (-)-hardwickiic acid (Compound 1) and (-)-abietic acid (Compound 2). The isolates were evaluated for their antimicrobial properties against several plant pathogenic bacterial and fungal strains. Both compounds demonstrated an antibacterial effect, especially against Gram-positive bacterial strains (Bacillus spizizenii, Clavibacter michiganensis subsp. michiganensis, and Curtobacterium flaccumfaciens pv. flaccumfaciens) with half maximal inhibitory concentration (IC50) between 1 and 5.1 mu g/mL (5-20 times higher than that of the positive control gentamicin). In the used concentrations, minimal bactericidal concentration (MBC) was reached only against the non-pathogen B. spizizenii. Besides their activity against Fusarium avenaceum, the highest antifungal activity was observed for Compound 1 against Bipolaris sorokiniana with an IC50 of 3.8 mu g/mL.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available