4.6 Article

Exploration of the Transglycosylation Activity of Barley Limit Dextrinase for Production of Novel Glycoconjugates

Journal

MOLECULES
Volume 28, Issue 10, Pages -

Publisher

MDPI
DOI: 10.3390/molecules28104111

Keywords

glycoside hydrolase family 13; alpha-glucan debranching enzyme; CAZyme profiling; pullulan; cyclodextrins; glycosyl fluorides

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A study found that the enzyme HvLD from barley showed a preference for pNP maltoside as both acceptor and donor, and exhibited excellent activity when pullulan or its fragment was used as the donor. In addition, HvLD could use a-maltosyl fluoride as a donor and maltose as the best acceptor. The findings provide insights for the production of glycoconjugate compounds with novel glycosylation patterns using natural donors.
A few a-glucan debranching enzymes (DBEs) of the large glycoside hydrolase family 13 (GH13), also known as the a-amylase family, have been shown to catalyze transglycosylation as well as hydrolysis. However, little is known about their acceptor and donor preferences. Here, a DBE from barley, limit dextrinase (HvLD), is used as a case study. Its transglycosylation activity is studied using two approaches; (i) natural substrates as donors and different p-nitrophenyl (pNP) sugars as well as different small glycosides as acceptors, and (ii) a-maltosyl and a-maltotriosyl fluorides as donors with linear maltooligosaccharides, cyclodextrins, and GH inhibitors as acceptors. HvLD showed a clear preference for pNP maltoside both as acceptor/donor and acceptor with the natural substrate pullulan or a pullulan fragment as donor. Maltose was the best acceptor with a-maltosyl fluoride as donor. The findings highlight the importance of the subsite +2 of HvLD for activity and selectivity when maltooligosaccharides function as acceptors. However, remarkably, HvLD is not very selective when it comes to aglycone moiety; different aromatic ring-containing molecules besides pNP could function as acceptors. The transglycosylation activity of HvLD can provide glycoconjugate compounds with novel glycosylation patterns from natural donors such as pullulan, although the reaction would benefit from optimization.

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