Journal
MOLECULES
Volume 28, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/molecules28073172
Keywords
stereochemistry; 3-Br-acivicin; Plasmodium falciparum; glyceraldehyde 3-phosphate dehydrogenase; multitarget; covalent inhibitors
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This study investigated the significance of chirality for the natural compound 3-Br-acivicin (3-BA) and its derivatives. The (5S, aS) isomers displayed significant antiplasmodial activity, suggesting their uptake might be mediated by the L-amino acid transport system. Stereochemistry only affected target binding for two subclasses, but led to significant differences in antimalarial activity for all subclasses, indicating that stereoselective uptake might be responsible for the enhanced activity of (5S, aS) isomers.
Chiral natural compounds are often biosynthesized in an enantiomerically pure fashion, and stereochemistry plays a pivotal role in biological activity. Herein, we investigated the significance of chirality for nature-inspired 3-Br-acivicin (3-BA) and its derivatives. The three unnatural isomers of 3-BA and its ester and amide derivatives were prepared and characterized for their antimalarial activity. Only the (5S, aS) isomers displayed significant antiplasmodial activity, revealing that their uptake might be mediated by the L-amino acid transport system, which is known to mediate the acivicin membrane's permeability. In addition, we investigated the inhibitory activity towards Plasmodium falciparum glyceraldehyde 3-phosphate dehydrogenase (PfGAPDH) since it is involved in the multitarget mechanism of action of 3-BA. Molecular modeling has shed light on the structural and stereochemical requirements for an efficient interaction with PfGAPDH, leading to covalent irreversible binding and enzyme inactivation. While stereochemistry affects the target binding only for two subclasses (1a-d and 4a-d), it leads to significant differences in the antimalarial activity for all subclasses, suggesting that a stereoselective uptake might be responsible for the enhanced biological activity of the (5S, aS) isomers.
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