Journal
MOLECULES
Volume 28, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/molecules28114409
Keywords
Kampo; Kakkonto; anti-glycation activity; advanced glycation end products; ephedrine
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A total of 147 oral Kampo prescriptions were evaluated for their anti-glycation activity, and Kakkonto showed significant activity. Analysis of the chemical constituents of Kakkonto revealed the presence of various compounds, and further analysis identified ephedrine as the key component responsible for its anti-glycation activity. The extract of Ephedrae herba, which contains ephedrine, also exhibited strong anti-glycation activity.
A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine's contribution to Kakkonto's reactive carbonyl species' scavenging ability and anti-glycation activity.
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