Journal
MICROSCOPY AND MICROANALYSIS
Volume 29, Issue 3, Pages 1153-1167Publisher
OXFORD UNIV PRESS
DOI: 10.1093/micmic/ozad023
Keywords
angiogenesis; antitumor; apoptosis; light microscope; Polyalthia longifolia
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The study aimed to investigate the effects of Polyalthia longifolia leaf extract on the growth of HeLa cell xenograft tumor in nude mice and its mechanism. The results showed that the leaf extract effectively inhibited the growth of HeLa cell xenograft tumors in nude mice. This inhibition was associated with apoptosis induction, decreased proliferation of HeLa cells, and reduced angiogenesis in the tumors. The findings suggest that Polyalthia longifolia leaf extract has potential as an anti-cancer agent.
The present study investigated the effects of Polyalthia longifolia leaf extract against the growth of HeLa cell xenograft tumor in nude mice and its underlying mechanism. The nude mice xenografted with HeLa cells were treated with 5% DMSO (vehicle control), 20 mg/kg/body weight of etoposide (positive control), and 500 and 1000 mg/kg/body weight of leaf extract, respectively. Antitumor activity was evaluated with apoptosis, proliferation, and angiogenesis using microscopic-based histological and immunohistochemical microanalyses. The tumor tissue histological and immunohistochemical analyses showed that the HeLa tumor cell death was associated with apoptosis and decreased (p < 0.05) expression of Ki-67 in tumor tissues. The extract also inhibits tumor angiogenesis by downregulating (p < 0.05) the expression of VEGF and CD31 in tumor tissues after treatment for 35 days. Conclusively, the P. longifolia leaf extract effectively inhibited HeLa cell xenograft growth in nude mice. The possible mechanism was related to induction of apoptosis, inhibition of tumor HeLa cell proliferation by decreasing the Ki-67 protein expression, and prevention of tumor angiogenesis by reducing VEGF and CD31 protein expression in HeLa cells.
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