Characterization, estimation of virulence and drug resistance of diarrheagenic escherichia coli (DEC) isolated from Tibetan pigs

In this study, we collected feces of Tibetan piglets from Nyingchi area for isolation, culture, identification, virulence gene analysis and drug resistance analysis of Escherichia Coli. The results demonstrated a 41.3% isolation rate of Diarrheagenic Escherichia Coli from Tibetan pigs with the main phylogenetic groups: group A (68.6%) and group B2 (15.7%). Typical E.coli accounted for 76.5%. The highest detection rates of porcine virulence genes were E.coli heat-resistant enterotoxin STb (58.82%) and F107 fimbrial subunit (23.53%). The highest detection rates of virulence genes from Tibetan pigs were fimC (80.39%) and ompA (76.47%). A drug sensitivity test showed that Diarrheagenic Escherichia Coli from Tibetan pigs had high drug resistance rates to mezlocillin, doxycycline and gentamicin. This study comprehensively analyzed the species composition, virulence and drug resistance of Diarrheagenic Escherichia Coli from Tibetan pigs, which provided a clearer and more targeted idea for the prevention and treatment of yellow and white dysentery in Tibetan pigs in the future.

Automated summary

In this study, fecal samples of Tibetan piglets from Nyingchi area were analyzed for the presence of Diarrheagenic Escherichia Coli. The isolation rate was found to be 41.3%, with the predominant phylogenetic groups being group A (68.6%) and group B2 (15.7%). The highest detection rates of virulence genes were STb and F107 in E.coli, and fimC and ompA in Tibetan pigs. Drug sensitivity testing revealed high resistance rates to mezlocillin, doxycycline, and gentamicin. This study provides important insights for the prevention and treatment of yellow and white dysentery in Tibetan pigs.