Antiproliferative activity of secondary metabolites isolated from Humboldtia unijuga Bedd.

Humboldtia unijuga Bedd. (Fabaceae), a tree species endemic to southern region of Western Ghats in India, is least explored for its chemical constituents and biological activities. Phytochemical analysis of H. unijuga roots and stems resulted in the isolation of twenty-nine secondary metabolites with four new molecules, viz., methyl 3-(tetradecanoyloxy)olean-12-en-28-oate (3), lup-20(29)-en-3-yl 3-hydroxyheptadecanoate (26), methyl 3-(dodecanoyloxy)olean-12-en-28-oate (27), 28-oxoolean-12-en-3-yl myristate (28). Antiproliferative (cytotoxicity) activity of the twenty-nine isolated compounds was evaluated by MTT assay in five cancer cell lines, viz., C6, Hepa 1-6, RIN-5F, DLA and PC-12. These in vitro screening assays revealed promising anticancer activity of oleanolic acid-3-acetate (5) and (2S)-1-O-palmitoyl-2-O-(9Z-octadecenoyl)-3-O-(6'-sulfo-a-D-quinovopyranosyl) glycerol (16). The mechanism of anticancer activity of these two molecules was elucidated by phase contrast and fluorescent microscopy, caspase 3 activity assay and flow cytometric cell cycle/apoptotic analyses. These compounds demonstrated anticancer activity on C6 and DLA cells by inducing cell specific apoptosis. Briefly, this study led to the isolation of twenty nine secondary metabolites, with four new ones, from H. unijuga, and two of the isolated molecules demonstrated promising anticancer activity.

Automated summary

Humboldtia unijuga Bedd., an endemic tree species in the southern region of Western Ghats in India, remains poorly explored for its chemical constituents and biological activities. This study identified twenty-nine secondary metabolites, including four new molecules, from the roots and stems of H. unijuga. Among them, oleanolic acid-3-acetate and (2S)-1-O-palmitoyl-2-O-(9Z-octadecenoyl)-3-O-(6'-sulfo-α-D-quinovopyranosyl) glycerol exhibited promising anticancer activity by inducing cell-specific apoptosis in C6 and DLA cells.