Identification and Genomic Localization of Autosomal sdY Locus in a Population of Atlantic Salmon (Salmo salar)

The determination of sex in salmonid fishes is controlled by genetic mechanisms, with males being the heterogametic sex. The master sex-determining gene, the sexually dimorphic gene on the Y chromosome (sdY), is a conserved gene across various salmonid species. Nevertheless, variations in the genomic location of sdY have been observed both within and between species. Furthermore, different studies have reported discordances in the association between the sdY and the phenotypic gender. While some males seem to lack this locus, there have been reports of females carrying sdY. Although the exact reasons behind this discordance remain under investigation, some recent studies have proposed the existence of an autosomal, non-functional copy of sdY as a potential cause. In this study, we confirmed the presence of this autosomal sdY in the SalmoBreed strain of Atlantic salmon using a genotyping platform through a novel approach that allows for high-throughput screening of a large number of individuals. We further characterized the segregation profile of this locus across families and found the ratio of genetically assigned female-to-male progeny to be in accordance with the expected profile of a single autosomal sdY locus. Additionally, our mapping efforts localized this locus to chromosome 3 and suggested a putative copy on chromosome 6.

Automated summary

Sex determination in salmonid fishes is regulated by genetic mechanisms, with males being heterogametic. The master sex-determining gene, sdY, shows variations in genomic location among and within species. Discordances in the association between sdY and gender have been reported, with some males lacking this locus and reports of females carrying sdY. This study confirmed the presence of an autosomal sdY copy in Atlantic salmon and found its localization on chromosome 3 and a putative copy on chromosome 6.