The role of root size and root efficiency in grain production, and water-and nitrogen-use efficiency in wheat

BACKGROUNDThe root system is the major plant organ involved in water and nutrient acquisition, influencing plant growth and productivity. However, the relative importance of root size and uptake efficiency remains undetermined. A pot experiment was conducted using two wheat varieties with different root sizes to evaluate their capacity for water and nitrogen (N) uptake and their effects on grain production, water-use efficiency (WUE), and N-use efficiency (NUE) under two water treatments combined with three N levels. RESULTSThe leaf water potential and root exudates of changhan58 (CH, small root variety) were higher or similar to those of changwu134 (CW, large root variety) under water/N treatment combinations, indicating that small roots can transport enough water to above the ground. The addition of N improved plant growth, photosynthetic traits, and WUE significantly. There were no significant differences in WUE or grain production between the two cultivars under well-watered conditions. However, they were significantly higher in CH than in CW under water deficit stress. Nitrogen uptake per unit root dry weight, glutaminase, and nitrate reductase activities were significantly higher in CH than in CW, regardless of moisture conditions. Root biomass was positively correlated with evapotranspiration, while the root/shoot ratio was negatively correlated with WUE (P < 0.05) but not with NUE. CONCLUSIONIn a pot experiment, water and N uptake were more strongly associated with resource uptake availability than root size. This may provide guidance in wheat breeding programs for drought-prone regions. & COPY; 2023 Society of Chemical Industry.

Automated summary

In this experiment, the water and nitrogen uptake of two wheat varieties with different root sizes were studied, as well as their effects on grain production, water-use efficiency and nitrogen-use efficiency. The results showed that the small root variety could transport enough water to the aboveground part, and nitrogen fertilization significantly improved plant growth and water-use efficiency. Under water deficit stress, the small root variety exhibited higher water-use efficiency and grain production. Nitrogen uptake, glutaminase, and nitrate reductase activities were higher in the small root variety.