Development of a Chemogenetic Approach to Manipulate Intracellular pH

Chemogenetic Operation of iNTRacellular prOton Levels(pH-Control)is a novel substrate-based enzymatic method that enables precise spatiotemporalcontrol of ultralocal acidification in cultured cell lines and primaryneurons. The genetically encoded biosensor SypHer3s showed that pH-Controleffectively acidifies cytosolic, mitochondrial, and nuclear pH exclusivelyin the presence of beta-chloro-d-alanine in living cellsin a concentration-dependent manner. The pH-Control approach is promisingfor investigating the ultralocal pH imbalance associated with manydiseases.

Automated summary

pH-Control is a novel enzymatic method that allows precise control of ultralocal acidification in living cells. The genetically encoded biosensor SypHer3s demonstrated that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH in a concentration-dependent manner. This approach shows promise in investigating the ultralocal pH imbalance associated with various diseases.