Journal
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume 11, Issue 8, Pages 2250-2260Publisher
WILEY
DOI: 10.1002/term.2123
Keywords
neural scaffolds; extracellular matrix (ECM); bone marrow mesenchymal stem cells (BMSCs); chitosan; silk fibroin; peripheral nerve repair
Categories
Funding
- National Key Basic Research Programme of China [2014CB542202]
- 863 Programme [2012AA020502]
- National Natural Science Foundation of China [81200932, 81471255]
- Natural Science Foundation of Jiangsu Province [BK2012658]
- Basic Research Programme of Jiangsu Education Department [14KJA310004]
- Priority Academic Programme Development of Jiangsu Higher Education Institutions (PAPD)
Ask authors/readers for more resources
To advance molecular and cellular therapy into the clinic for peripheral nerve injury, modification of neural scaffolds with the extracellular matrix (ECM) of peripheral nerves has been established as a promising alternative to direct inclusion of support cells and/or growth factors within a neural scaffold, while cell-derived ECM proves to be superior to tissue-derived ECM in the modification of neural scaffolds. Based on the fact that bone marrow mesenchymal stem cells (BMSCs), just like Schwann cells, are adopted as support cells within a neural scaffold, in this study we used BMSCs as parent cells to generate ECM for application in peripheral nerve tissue engineering. A chitosan nerve guidance conduit (NGC) and silk fibroin filamentous fillers were respectively prepared for co-culture with purified BMSCs, followed by decellularization to stimulate ECM deposition. The ECM-modified NGC and lumen fillers were then assembled into a chitosan-silk fibroin-based, BMSC-derived, ECM-modified neural scaffold, which was implanted into rats to bridge a 10mm-long sciatic nerve gap. Histological and functional assessments after implantation showed that regenerative outcomes achieved by our engineered neural scaffold were better than those achieved by a plain chitosan-silk fibroin scaffold, and suggested the benefits of BMSC-derived ECM for peripheral nerve repair. Copyright (c) 2016 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available