4.7 Article

Massive Proteogenomic Reanalysis of Publicly Available Proteomic Datasets of Human Tissues in Search for Protein Recoding via Adenosine-to-Inosine RNA Editing

Journal

JOURNAL OF PROTEOME RESEARCH
Volume 22, Issue 6, Pages 1695-1711

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.2c00740

Keywords

RNA editing; ADAR; proteogenomics; human proteome; IGFBP7

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The proteogenomic search pipeline was used to reanalyze 40 publicly available shotgun proteomic datasets from various human tissues. A total of 5442 .raw data files were processed, identifying 33 recoded protein sites in 21 datasets. The study demonstrated that neural and cancer tissues were enriched with recoded proteins and the recoding rate was regulated by the interaction of enzymes with mRNA.
The proteogenomic search pipeline developed in this work has been applied for reanalysis of 40 publicly available shotgun proteomic datasets from various human tissues comprising more than 8000 individual LC-MS/MS runs, of which 5442 .raw data files were processed in total. This reanalysis was focused on searching for ADAR-mediated RNA editing events, their clustering across samples of different origins, and classification. In total, 33 recoded protein sites were identified in 21 datasets. Of those, 18 sites were detected in at least two datasets, representing the core human protein editome. In agreement with prior artworks, neural and cancer tissues were found to be enriched with recoded proteins. Quantitative analysis indicated that recoding the rate of specific sites did not directly depend on the levels of ADAR enzymes or targeted proteins themselves, rather it was governed by differential and yet undescribed regulation of interaction of enzymes with mRNA. Nine recoding sites conservative between humans and rodents were validated by targeted proteomics using stable isotope standards in the murine brain cortex and cerebellum, and an additional one was validated in human cerebrospinal fluid. In addition to previous data of the same type from cancer proteomes, we provide a comprehensive catalog of recoding events caused by ADAR RNA editing in the human proteome.

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