UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications

Real-time breath analysis using secondary electro-spray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to access the metabolic state of a person. However, it lacks the ability to unequivocally assign mass spectral features to compounds due to the absence of chromato-graphic separation. This can be overcomed by using exhaled breath condensate and conventional liquid chromatography-mass spec-trometry (LC-MS) systems. In this study, to the best of our knowledge, we confirm for the first time the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr) previously reported to be involved in response to and side effects from antiseizure medications in exhaled breath condensate and by extension in exhaled human breath. Raw data are publicly available at MetaboLights with the accession number MTBLS6760.

Automated summary

Real-time breath analysis using secondary electro-spray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to assess a person's metabolic state. However, the inability to confidently assign mass spectral features to compounds due to the lack of chromatographic separation can be overcome by using exhaled breath condensate and conventional LC-MS systems. In this study, the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr), which have been reported to be involved in response to antiseizure medications and their side effects, was confirmed in exhaled breath condensate and, by extension, in exhaled human breath for the first time. Raw data is publicly available at MetaboLights with the accession number MTBLS6760.