Journal
JOURNAL OF PHYSICS D-APPLIED PHYSICS
Volume 56, Issue 29, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1361-6463/accc3d
Keywords
non-thermal plasma; oxidative stress; live-cell imaging; fluorescence imaging; phototoxicity; plasma sensitivity; cell passage
Categories
Ask authors/readers for more resources
Live-cell imaging with fluorescence microscopy is a powerful tool in cancer research but can result in phototoxicity, causing disruption of redox balance and an overload of reactive oxygen species. We investigated the impact of phototoxicity on cellular homeostasis and response to non-thermal plasma (NTP), revealing increased sensitivity to NTP in cells used for live-cell imaging, likely due to altered proliferation rates and ROS levels. These findings have important implications for research using this technique and evaluating redox-based therapies.
Live-cell imaging with fluorescence microscopy is a powerful tool, especially in cancer research, widely-used for capturing dynamic cellular processes over time. However, light-induced toxicity (phototoxicity) can be incurred from this method, via disruption of intracellular redox balance and an overload of reactive oxygen species (ROS). This can introduce confounding effects in an experiment, especially in the context of evaluating and screening novel therapies. Here, we aimed to unravel whether phototoxicity can impact cellular homeostasis and response to non-thermal plasma (NTP), a therapeutic strategy which specifically targets the intracellular redox balance. We demonstrate that cells incorporated with a fluorescent reporter for live-cell imaging have increased sensitivity to NTP, when exposed to ambient light or fluorescence excitation, likely through altered proliferation rates and baseline intracellular ROS levels. These changes became even more pronounced the longer the cells stayed in culture. Therefore, our results have important implications for research implementing this analysis technique and are particularly important for designing experiments and evaluating redox-based therapies like NTP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available