Journal
JOURNAL OF MOLECULAR LIQUIDS
Volume 376, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.molliq.2023.121476
Keywords
Lactic acid bacteria; Nitrate reductase; Optimization; Silver nanoparticles; Characterization; Antimicrobial
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The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of silver nanoparticles (AgNPs) and characterize the biosynthesized AgNPs. Lactobacillus plantarum CAM 4 produced higher nitrate reductase activity under optimized medium and successfully enhanced the biosynthesis of AgNPs. The biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens.
Among emerging nanotechnology, silver nanoparticles (AgNPs) get much attention due to their unique physicochemical properties. The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of AgNPs and characterize the biosynthesized AgNPs. Lactobacillus plantarum CAM 4 produced higher nitrate reductase activity (121.77 U/mL) under optimized medium containing 0.75 % of glucose, 2 % of KNO3, and pH 5.0 and successfully enhanced the AgNPs biosynthesis. Spectrophotometric analysis revealed that the biosynthesized AgNPs exhibited a peak at 430 nm. DLS analysis indicated the monodispersed characteristic of AgNPs. TEM and FE-SEM analysis shows that the AgNPs were spherical-like shape, while the EDX analysis spectrum revealed the existence of an Ag element in AgNPs. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens. Results from this study provide notable insights into sustainable and inexpensive methods for the enhanced production of AgNPs using LAB. (c) 2023 Elsevier B.V. All rights reserved.
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