Sinomonas terrae sp. nov., Isolated from an Agricultural Soil

While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5(T), was isolated. The cells of the strain were Gramstaining-positive, aerobic and non-motile rods. Growth occurred at 10- 42oC (optimum, 30oC), pH 5.59.0 (optimum, pH 7.0-7.5), and 0- 2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C15: 0 anteiso, C17: 0 anteiso, and summed feature 8 (C18: 1.7c and/or C18: 1.6c). The predominant menaquinone was MK-9 (H-2). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5(T) is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117(T), sharing a genetic similarity of 98.4%. The draft genome of strain 5-5(T) was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5(T) was 68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5(T) and its closest strains S. humi MUSC 117(T) and S. susongensis A31(T) were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5(T) and its closest strains S. humi MUSC 117(T) and S. susongensis A31(T) were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA- DNA hybridization analyses, the 55(T) strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5(T) (=KCTC 49650(T)=NBRC 115790(T)).

Automated summary

A novel bacterial strain named 5-5(T) was isolated during the search for pesticide-degrading bacteria in soybean field soil. The strain belonged to the genus Sinomonas and had a genetically similarity of 98.4% with Sinomonas humi MUSC 117(T). It was identified as a new species, Sinomonas terrae sp. nov., based on phenotypic, genotypic, and chemotaxonomic analyses.