Description of Luteibacter aegosomatis sp. nov., Luteibacter aegosomaticola sp. nov., and Luteibacter aegosomatissinici sp. nov. isolated from the Intestines of Aegosoma sinicum Larvae

Three novel bacterial strains, 321(T), 335(T), and 353(T), were isolated from the intestines of Aegosoma sinicum larvae collected from Paju-Si, South Korea. The strains were Gram-negative, obligate aerobe and had rod-shaped cells with a single flagellum. The three strains belonged to the genus Luteibacter in the family Rhodanobacteraceae and shared < 99.2% similarity in their 16S rRNA gene sequence and < 83.56% similarity in thier whole genome sequence. Strains 321(T), 335(T), and 353(T) formed a monophyletic clade with Luteibacter yeojuensis KACC 11405(T), L. anthropi KACC 17855(T), and L. rhizovicinus KACC 12830(T), with sequence similarities of 98.77-98.91%, 98.44-98.58%, and 97.88-98.02%, respectively. Further genomic analyses, including the construction of the Up-to-date Bacterial Core Gene (UBCG) tree and assessment of other genome-related indices, indicated that these strains were novel species belonging to the genus Luteibacter. All three strains contained ubiquinone Q8 as their major isoprenoid quinone and iso-C-15:0 and summed feature 9 (C-16:0 10-methyl and/or iso-C-17:1 omega 9c) as their major cellular fatty acids. Phosphatidylethanolamine and diphosphatidylglycerol were the major polar lipids in all the strains. The genomic DNA G + C contents of strains 321(T), 335(T), and 353(T) were 66.0, 64.5, and 64.5 mol%, respectively. Based on multiphasic classification, strains 321(T), 335(T), and 353(T) were classified into the genus Luteibacter as the type strains of novel species, for which the names Luteibacter aegosomatis sp. nov., Luteibacter aegosomaticola sp. nov., and Luteibacter aegosomatissinici sp. nov. are proposed, respectively.

Automated summary

Three new bacterial strains, 321(T), 335(T), and 353(T), were isolated from the intestines of Aegosoma sinicum larvae collected in Paju-Si, South Korea. These strains belong to the genus Luteibacter and are characterized as Gram-negative, obligate aerobes with rod-shaped cells and a single flagellum. Through genomic analysis, it is determined that these strains are novel species within the genus Luteibacter.