Journal
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
Volume 33, Issue 5, Pages 1388-1399Publisher
SPRINGER
DOI: 10.1007/s10904-023-02592-1
Keywords
Sensors; Quantum Dots; Photoluminescence; ZnS; Glutathione; Urease
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ZnS quantum dots (QDs) capped with glutathione (GSH) were synthesized and characterized. The GSH-capped ZnS QDs showed potential as optical probes for non-enzymatic and enzymatic detection of urea, with improved sensitivity and applicability achieved through enzyme conjugation.
Quantum dots (QDs) possess characteristic chemical and optical features. In this light, ZnS QDs capped with glutathione (GSH) were synthesized via an easy aqueous co-precipitation technique. Fabricated QDs were characterized in terms of X-ray diffraction (XRD), high resolution transmission electron microscope (HRTEM), Fourier transform infrared (FTIR) and Zeta potential analyses. Optical properties were examined using photoluminescence (PL) and ultraviolet-visible (UV-visible) spectroscopies. Moreover, GSH-capped ZnS QDs were evaluated as an optical probe for non-enzymatic detection of urea depending on the quenching of PL intensity of ZnS QDs in the presence of urea from concentration range of 0.5-5 mM with a correlation coefficient (R-2) of 0.995, sensitivity of 0.0875 mM(-1) and LOD of 0.426 mM. Furthermore, GSH-capped ZnS QDs-urease conjugate was utilized as an optical probe for enzymatic detection of urea in the range from 1.0 mu M to 5.0 mM. Interestingly, it was observed that urea has a good affinity towards ZnS QDs-urease conjugate with a linear relationship between the change of PL intensity and urea concentration. It was found that R-2 is 0.997 with a sensitivity of 0.042 mM(-1) for mM concentration (0.5-5 mM) and LOD of 0.401 mM. In case of mu M concentration range (1-100 mu M), R-2 was 0.971 with a sensitivity of 0.0024 mu M-1 and LOD of 0.687 mu M. These data suggest that enzyme conjugation to capped QDs might improve their sensitivity and applicability. [GRAPHICS] .
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