The Type VI Secretion System Contributes to the Invasiveness of Liver Abscess Caused by Klebsiella pneumoniae

Background Klebsiella pneumoniae liver abscess (KPLA) with extrahepatic migratory infections is defined as invasive KPLA (IKPLA). The type VI secretion system (T6SS) is involved in the pathogenesis of KPLA. We hypothesized that T6SS plays a role in IKPLA. Methods 16S ribosomal RNA gene sequencing was performed on abscess samples. Polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) was used to validate the expression difference of T6SS hallmark genes. In vitro and in vivo experiments were performed to identify the pathogenic feature of T6SS. Results PICRUSt2 predicted that the T6SS-related genes were notably enriched in the IKPLA group. PCR detection of T6SS hallmark genes (hcp, vgrG, and icmF) showed that 197 (81.1%) were T6SS-positive strains. The T6SS-positive strain detection rate in the IKPLA group was higher than in the KPLA group (97.1% vs 78.4%; P < .05). RT-PCR showed that the hcp expression level was markedly increased in IKPLA isolates (P < .05). The T6SS-positive isolates showed higher survival against serum and neutrophil killing (all P < .05). The T6SS-positive K pneumoniae-infected mice had a shorter survival time, higher mortality, and an increased interleukin 6 expression in the liver and lungs (all P < .05). Conclusions T6SS is an essential virulence factor for K pneumoniae and contributes to IKPLA. The type VI secretion system is an important virulence factor of liver abscess-associated Klebsiella pneumoniae and involved in the pathogenic process of invasive K pneumoniae liver abscess.

Automated summary

By analyzing abscess samples, the study found that the type VI secretion system (T6SS) is involved in the pathogenesis of invasive Klebsiella pneumoniae liver abscess (IKPLA). PCR and RT-PCR experiments confirmed the expression difference of T6SS hallmark genes and identified the pathogenic feature of T6SS. The results suggest that T6SS is an essential virulence factor for K pneumoniae and contributes to IKPLA.