Related references
Note: Only part of the references are listed.
Review
Agriculture, Multidisciplinary
Lijuan Yin et al.
Summary: Foodborne viruses are recognized as significant threats to food safety and human health. Rapid and accurate detection is crucial for food safety control. This review focuses on the progress of detection methods for foodborne viruses and summarizes current methods including electron microscopy, immunoassay, molecular technology, biosensors, and CRISPR/Cas-based detection technology.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Environmental
Tao Yang et al.
Summary: In this study, a hydrogel-mediated RT-LAMP method was developed for rapid and accurate quantification of SARS-CoV-2 virus. The technique offers fast, simple, and highly sensitive detection, and can be used for monitoring cold chain foods.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Virology
Yaru Li et al.
Summary: The pandemic caused by SARS-CoV-2 has significantly affected the global economy. While the virus mainly spreads through human-to-human transmission, there is a possibility of transmission through cold-chain foods and packaging. It is crucial to develop countermeasures and surveillance methods for detecting SARS-CoV-2 in these specific areas.
Article
Engineering, Environmental
Long Ma et al.
Summary: We developed a versatile biosensing platform, SCENT-Cas (Silver nanoCluster Empowered Nucleic acids Test using CRISPR/Cas12a), for ultrasensitive detection of pathogenic bacteria. This platform used the isothermal amplification of the invA gene of Salmonella typhimurium to trigger trans-cleavage of CRISPR/Cas12a. The trans-cleavage was then utilized to degrade single-stranded DNA and create a ratiometric fluorescence biosensing platform. This method enabled the detection of 1 CFU/mL S. typhi with a dynamic range from 1 to 108 CFU/mL, and showed promise for real food sample testing.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Yu Wang et al.
Summary: A CRISPR-Cas12a-based fluorescence/colorimetric biosensor was developed for efficient detection of small molecules and protein targets, with significant implications in the fields of food safety, environmental monitoring, and clinical diagnosis.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biophysics
Quynh Anh Phan et al.
Summary: CRISPR-Dx offers broad enhancements compared to traditional nanobiosensors by utilizing the trans-cleavage activity of the CRISPR/Cas systems. However, the current singlestranded DNA/RNA reporters of CRISPR-Dx have poor stability and limited sensitivity. Research is shifting towards using the trans-cleavage activity of CRISPR/Cas effectors on metal nanoparticles to develop novel nanobiosensors with high accuracy, sensitivity, selectivity, and versatility.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Kai Zhang et al.
Summary: This study successfully constructed a method for SARS-CoV-2 nucleic acid detection using an exonuclease III cleavage reaction-based isothermal amplification combined with a pH-induced regenerative ECL biosensor mediated by CRISPR/Cas12a, providing ultra-sensitive and specific detection capabilities essential for large-scale screening in resource-limited areas.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Materials Science, Multidisciplinary
Li Liu et al.
Summary: A gold nanoparticle-labeled CRISPR-Cas13a nucleic acid assay has been developed for sensitive solid-state nanopore sensing. This simplified assay enables rapid and portable nucleic acid biomarker detection without relying on thermal cyclers.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biophysics
Long Ma et al.
Summary: Researchers have developed a CRISPR-Cas12a powered biosensor for ultra-sensitive and selective detection of SARS-CoV-2, with smartphone readout capability. The biosensor showed excellent performance in detecting synthetic vectors, transcribed RNA, and pseudoviruses, providing 100% agreement with qPCR results when tested with clinical samples. It offers a novel and robust technology for clinically sensitive detection of SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Siwenjie Qian et al.
Summary: This article reviews the recent advances of CRISPR-based amplification-free methods for nucleic acid detection, discusses the pros and cons of these methods, and further explores the challenges including sample preparation, off-target effect, sequences limit, quantitative and multiplex detection. It highlights the potential of CRISPR-powered detection methods to pave the way for rapid, cheap, highly sensitive and specific on-site detection without amplification.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biophysics
Qin Zhang et al.
Summary: The development of reliable and fast diagnostic devices for COVID-19 is crucial. In this study, a new analysis principle combining reverse transcription and isothermal amplification with CRISPR/Cas13a reaction was proposed. The method showed good sensitivity and specificity in detecting the SARS-CoV-2 gene fragment, and results could be obtained within 1 hour using a portable device. This approach has the potential for point-of-care screening in remote areas with limited resources.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Multidisciplinary
Olga Guselnikova et al.
Summary: This article reviews recent fabrication methods for surface-enhanced Raman spectroscopy (SERS) substrates, focusing on advanced nanoarchitecture based on noble metals, nanolayered 2D materials, and nanoarchitecturing with organic molecules. The use of SERS has been extensively investigated for its enhanced signal intensity and the ability to detect a very small number of molecules. Nanoarchitecture strategies are crucial in the development of SERS substrates. The article discusses recent achievements in the morphology of metallic nanostructures and outlines future directions for reproducible preparation and well-controlled nanoarchitecture. The use of nanolayered 2D materials as an alternative to noble metals and the hybridization with organic molecules to improve stability and selectivity of SERS sensing are also described.
Article
Engineering, Biomedical
Shuhan Lu et al.
Summary: This study presents a one-step fluorescence assay for the detection of SARS-CoV-2 RNA in nasopharyngeal samples. The assay offers a short detection time and sensitivity comparable to RT-qPCR, while also allowing for flexible assay design.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Chemistry, Analytical
Diego A. Huyke et al.
Summary: This study quantifies the kinetic parameters and detection limits of enzymes in CRISPR-Cas12 and CRISPR-Cas13 detection, and validates the self-consistency of the measurements. The results indicate that successful target detection requires the cleavage of at least 0.1% of the fluorescent reporter molecules, which is independent of the detection method and detector sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jianwen Zhuang et al.
Summary: In this study, a RPA-Cas12a-mu PAD was designed using CRISPR/Cas12a and SERS technology for highly sensitive bacterial detection. The concentration of S.typhi was determined by the aggregation level of SERS nanoprobes, enabling fast and accurate testing of food samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Fei Deng et al.
Summary: This study demonstrates the chemically enhanced trans-cleavage activity of Cas12a and Cas13a nucleases, leading to improved performance of CRISPR/Cas biosensors. Sulfhydryl reductants and non-ionic surfactants were found effective in enhancing the trans-ssDNA cleavage of Cas12a and trans-ssRNA cleavage of Cas13a, respectively. The mechanism of enhancement was identified to be a conformational change of the ribonucleoprotein, resulting in a significant increase in the relevant intramolecular distance. These chemical enhancers were easily integrated into existing CRISPR/Cas biosensing protocols without additional modifications. The detection of Helicobacter Pylori DNA and SARS-CoV-2 RNA showed significantly reduced reaction time and increased sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xueping Yang et al.
Summary: CRISPR/Cas-based systems have intrinsic merits for developing diagnostic technologies, but nucleic acid amplification is still required for high sensitivity. This study presents a one-step and amplification-free detection method using Cas12a-based assay and a single-microbead detection platform, which allows single-molecule level detection of DNA. The method has been successfully applied for specific identification in real samples without extra nucleic acid amplification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Noor Mohammad et al.
Summary: This study demonstrates a novel CRISPR-Cas12a-based sensing platform for detecting single-stranded DNA targets by quantifying the size reduction of double-stranded DNA as report molecules. It is simple, inexpensive, non-optical, and exhibits high sensitivity compared to fluorescent methods.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biophysics
Yangdao Wei et al.
Summary: A new platform for the detection of live Staphylococcus aureus (S. aureus) based on an Aptamer-based Cas14a1 Biosensor (ACasB) has been developed. This platform shows high specificity and sensitivity, and can be applied in food safety and environmental monitoring.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Jisui Tan et al.
Summary: In this study, a portable nuclear receptor-based biosensor was developed for highly sensitive analysis of a wide range of potential endocrine-disrupting chemicals (EDCs) in environmental water samples. The biosensor showed a detection limit of 5 ng/L E-2-binding activity equivalent (E-2-BAE) and 93 ng/L T3-BAE. The biosensor was used to map the estrogenic binding activities of surface waters from a rural community in the Yellow River basin in China, and a high correlation was observed when compared with the traditional yeast two-hybrid bioassay.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Rui Wang et al.
Summary: The proposed method integrates rapid PCR amplification and Cas12a cleavage, simplifying the operation and avoiding contamination. It demonstrates high detection sensitivity and reliability, and has broad application prospects in fields such as food safety and clinical diagnosis, offering a potential solution for rapid on-site testing.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Microbiology
Ben Hu et al.
Summary: This review summarizes the research progress on SARS-CoV-2 and COVID-19, including virology characteristics, pathogenesis, and recent advances in treatment methods. Furthermore, it discusses in detail the potential wildlife hosts and zoonotic origin of this emerging virus.
NATURE REVIEWS MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tian Tian et al.
Summary: The confinement effect-inspired Cas13a assay for single-molecule RNA diagnostics eliminates the need for nucleic acid amplification and reverse transcription, achieving a >10,000-fold enhancement in sensitivity and enabling absolute digital single-molecule RNA quantitation. Its broad applicability and accurate detection of various RNA molecules at the single-molecule level make it a potential dominant rival to RT-qPCR.
Article
Biophysics
Yi-Chen Du et al.
Summary: A highly sensitive biosensing strategy utilizing TdT and CRISPR-Cas12a for signal amplification was reported, showing great potential in the design of various biosensors and achieving ultrasensitive detection of UDG and T4 PNK. The biosensors developed based on this strategy display high sensitivity, signal amplification efficiency, and low background noise, making them promising for clinical diagnosis and biomedical research.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Parinaz Fozouni et al.
Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.
Article
Biophysics
Faezeh Ghorbanizamani et al.
Summary: The novel protein detection platform utilizes advanced technology with higher sensitivity, faster turnaround, and better stability compared to traditional nanoparticle methods. Smartphone-assisted sensing technology enables the tool to be used at home, reducing the burden on healthcare facilities.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Yihan Yang et al.
Summary: This study presents a rapid, easy-to-implement, and accurate biosensing platform for detecting SARS-CoV-2 using CRISPR technology. By combining Cas13a collateral effect with HCR reaction, the method shows good discrimination capability when target RNA of SARS-CoV-2 is added to negative oropharyngeal swabs, demonstrating its promising potential for point-of-care diagnosis of COVID-19.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Lijuan Yin et al.
Summary: This article highlights the recent advances in virus detection using CRISPR-Cas systems, particularly CRISPR-Cas12a/Cas13a. These systems, known for their sensitivity, specificity, high base resolution, and programmability, are revolutionizing virus detection and offering new possibilities in the field.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Jin-Ha Choi et al.
Summary: A non-nucleic acid amplification fluorescent biosensor using gold nanoparticles for metal-enhanced fluorescence detection of cfDNA has been developed in this study, allowing rapid and highly sensitive detection of BRCA-1. This sensor is highly selective and can be widely applied for the measurement of other nucleic acid biomarkers.
Article
Multidisciplinary Sciences
Bo Ning et al.
Summary: A portable and ultrasensitive saliva-based COVID-19 assay with a 15-minute sample-to-answer time has been developed, utilizing CRISPR-Cas12a activity to enhance viral amplicon signal without requiring RNA isolation or laboratory equipment. The device can quantify viral load over a broad linear range and has a lower limit of detection compared to the RT-PCR reference assay, showing potential for saliva-based point-of-care COVID-19 diagnosis.
Article
Chemistry, Analytical
Yuxi Wang et al.
Summary: The detection method using light-up CRISPR-Cas13 technology can sensitively and accurately detect SARS-CoV-2 and its mutated variants, with high specificity and sensitivity, allowing for strict discrimination of key mutations.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Peng-Fei Liu et al.
Summary: A novel Cas12a-based electrochemiluminescence biosensor was developed for target amplification-free HPV-16 DNA detection, with a detection limit of 0.48 pM and completion within 70 min. This unique combination of Cas12a with ECL technique shows promise for future applications in sensing analysis and diagnostic technologies beyond traditional CRISPR/Cas detection systems.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Environmental
S. Venkata Mohan et al.
Summary: The global spread of SARS-CoV-2 has had distressing effects on human health, economy, and ecosystem services. The potential fecal-oral transmission route is emerging as a major concern for community transmission, raising the need for further research and understanding to combat viral spread.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Lijuan Yin et al.
Summary: A G-quadruplex-based CRISPR-Cas12a bioassay was developed for high sensitivity and visualization detection of Salmonella. The method achieved a detection limit of 1 CFU/mL for Salmonella and successfully detected the bacteria in real food samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Yanju Chen et al.
Summary: qPCR gray zone testing results in uncertainty, while CRISPR technology can enhance detection signal sensitivity. A CRISPR-based checking method is proposed to solve gray zone problems, effectively eliminating uncertainty in sample classification without increasing workload in PCR detection.
Article
Biology
Hajime Shinoda et al.
Summary: The study introduces a novel CRISPR-based platform called SATORI, which allows amplification-free digital RNA detection with high sensitivity and specificity in a short period of time. The simultaneous use of multiple guide RNAs enhances sensitivity, enabling the detection of SARS-CoV-2 RNA at low levels.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Jianghua Liu et al.
Summary: Surface-enhanced Raman scattering (SERS) is a powerful tool for biosensors due to its ultrahigh sensitivity and unique fingerprint information. This study proposed a sensitive on-site nucleic acid detection strategy based on CRISPR/Cas12a with trans-cleavage activity on ssDNA linkers utilized to capture liposomes, enabling dual-mode detection of target DNA with SERS and naked eye.
ANALYTICAL CHEMISTRY
(2021)
Article
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Jiajie Liang et al.
Summary: S-CRISPR is a novel diagnostic platform without amplification, which can rapidly and sensitively detect SARS-CoV-2 RNA, showing potential as a strategy for rapid nucleic acid testing.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Long Ma et al.
Summary: The study proposed a CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria, with satisfactory selectivity for Salmonella and capabilities in food sample detection. The technology expands the reach of CRISPR-Cas system in biosensing and provides a general method for bacteria sensing with desirable sensitivity, specificity, and cross-validating capacity.
Article
Multidisciplinary Sciences
Helena de Puig et al.
Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.
Article
Materials Science, Multidisciplinary
Filipe S. R. Silva et al.
Summary: This study introduces an amplification-free CRISPR/Cas12a-based technology for SARS-CoV-2 RNA detection, with results readout using a smartphone camera. The method achieves high analytical sensitivity and accuracy, with results obtained in approximately 71 minutes.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Review
Engineering, Biomedical
Michael M. Kaminski et al.
Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
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Kai Shi et al.
Summary: CRISPR-Cas-only amplification network (CONAN) utilizes a Cas12a-driven reaction network to construct a positive feedback circuit, enabling isothermally amplified detection of genomic DNA with attomolar sensitivity, showcasing its potential as a powerful tool for disease diagnostics.
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