Uptake, translocation and subcellular distribution of broflanilide, afidopyropen, and flupyradifurone in mustard (Brassica juncea)

Novel pesticides broflanilide (BFI), afidopyropen (ADP), and flupyradifurone (FPO) have been widely used and become the new organic pollutants. However, uptake, translocation and residual distribution of BFI, ADP, and FPO in plants remain unclear. Therefore, residues distribution, uptake, and translocation of BFI, ADP, and FPO were investigated in mustard field trials and hydroponic experiments. The field results indicated that the residues of BFI, ADP, and FPO were 0.001-1.87 mg/kg at 0-21 d and dissipated fast in mustard (half-lives=5.2-11.3 d). More than 66.5 % of FPO residues were distributed in the cell-soluble fractions because of their high hydro-philicity, while hydrophobic BFI and ADP were primarily stored in the cell walls and organelles. The hydroponic data showed that the foliar uptake rates of BFI, ADP, and FPO were weak (bioconcentration factors<1), but the root uptake rate was strong (bioconcentration factors>1). The upward and downward translations of BFI, ADP, and FPO were limited (translation factor<1). BFI and ADP are uptake by roots via apoplast pathway, and FPO is uptake via symplastic pathway. This study contributes to the understanding of the formation of pesticide residues in plants and provides a reference for safe application and risk assessment of BFI, ADP, and FPO.

Automated summary

The uptake, translocation and residual distribution of novel pesticides BFI, ADP, and FPO in plants were investigated. The residues of BFI, ADP, and FPO in mustard dissipated quickly and more than 66.5% of FPO residues were distributed in the cell-soluble fractions. The foliar uptake rates of BFI, ADP, and FPO were low, but the root uptake rate was high. The study provides insight into the formation of pesticide residues in plants and offers guidance for the safe application and risk assessment of BFI, ADP, and FPO.