Phenomic data-driven biological prediction of maize through field-based high-throughput phenotyping integration with genomic data

High-throughput phenotyping (HTP) has expanded the dimensionality of data in plant research; however, HTP has resulted in few novel biological discoveries to date. Field-based HTP (FHTP), using small unoccupied aerial vehicles (UAVs) equipped with imaging sensors, can be deployed routinely to monitor segregating plant population interactions with the environment under biologically meaningful conditions. Here, flowering dates and plant height, important phenological fitness traits, were collected on 520 segregating maize recombinant inbred lines (RILs) in both irrigated and drought stress trials in 2018. Using UAV phenomic, single nucleotide polymorphism (SNP) genomic, as well as combined data, flowering times were predicted using several scenarios. Untested genotypes were predicted with 0.58, 0.59, and 0.41 prediction ability for anthesis, silking, and terminal plant height, respectively, using genomic data, but prediction ability increased to 0.77, 0.76, and 0.58 when phenomic and genomic data were used together. Using the phenomic data in a genome-wide association study, a heat-related candidate gene (GRMZM2G083810; hsp18f) was discovered using temporal reflectance phenotypes belonging to flowering times (both irrigated and drought) trials where heat stress also peaked. Thus, a relationship between plants and abiotic stresses belonging to a specific time of growth was revealed only through use of temporal phenomic data. Overall, this study showed that (i) it is possible to predict complex traits using high dimensional phenomic data between different environments, and (ii) temporal phenomic data can reveal a time-dependent association between genotypes and abiotic stresses, which can help understand mechanisms to develop resilient plants. Temporal phenotype data can predict complex traits of unknown genotypes in observed and unobserved environments as well as reveal the time-specific associations between genotypes and environmental stresses.

Automated summary

High-throughput phenotyping (HTP) has not resulted in many new biological discoveries, but field-based HTP (FHTP) using UAVs has the potential to monitor plant population interactions with the environment. This study collected phenotypic data on maize lines in different environments and predicted complex traits using genomic and phenomic data. The study revealed a time-dependent association between genotypes and abiotic stresses, highlighting the importance of temporal phenomic data.