Campomanesia lineatifolia Ruiz & Pavon (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation

Ethnopharmacological relevance: Campomanesia lineatifolia Ruiz & Pav ' on (Myrtaceae), an edible species found in Brazilian Forest, possesses leaves that are traditionally used for the treatment of gastrointestinal disorders in Brazil. Extracts of C. lineatifolia are rich in phenolics and exhibit antioxidant, and gastric antiulcer properties. Furthermore, Campomanesia spp. have been described to possess anti-inflammatory properties, but studies related to chemical constituents of C. lineatifolia are scarce in the literature. Aim of the study: This work aims to identify the chemical composition of the phenolic-rich ethanol extract (PEE) from C. lineatifolia leaves and evaluate the anti-inflammatory activity that could be related to its ethno-pharmacological use. Materials and methods: The high-speed countercurrent chromatography (HSCCC), using an isocratic and a step gradient elution method, and NMR, HPLC-ESI-QTOF-MS/MS were used to isolate and identify the chemicals of PEE, respectively. Lipopolysaccharide-(LPS)-stimulated THP-1 cells were used to evaluate the anti-inflammatory activities from PEE and the two majority flavonoids isolated by measure TNF-alpha and NF-kappa B inhibition assays. Results: Fourteen compounds were isolated from the PEE, further identified by NMR and HPLC-ESI-QTOF-MS/ MS, twelve of them are new compounds, and two others are already known for the species. The PEE, querci-trin and myricitrin promoted a concentration-dependent inhibition of TNF-alpha, and PEE promoted an inhibition of NF-kappa B pathway. Conclusions: PEE from C. lineatifolia leaves demonstrated significant anti-inflammatory activity that may be related to the traditional use to treat gastrointestinal disorders.

Automated summary

The leaves of Campomanesia lineatifolia, an edible plant in the Brazilian Forest, have been traditionally used for treating gastrointestinal disorders. The phenolic-rich ethanol extract (PEE) from C. lineatifolia leaves showed significant anti-inflammatory activity. The study aimed to identify the chemical composition of PEE and evaluate its potential in treating gastrointestinal disorders.