Photosensitizers with aggregation-induced far-red/near-infrared emission for versatile visualization and broad-spectrum photodynamic killing of pathogenic microbes

The exploration of photosensitizers with aggregation-induced emission (AIE PSs) for efficient visualization and broad-spectrum photodynamic killing of pathogenic microbes is a significant task. Herein, two far-red/near-infrared AIE-active PSs (TBTPy and TBTCy) were attained to show efficient Type I and Type II ROS generation, benefiting from the efficient ISC processes. The attained AIE PSs, especially TBTPy with bright emission, showed advantages in discriminating G+ bacteria over G- bacteria, and distinguishing dead E. coli from lived one. Both TBTPy and TBTCy have the capacity of broad-spectrum photodynamic killing of pathogenic microbes in vitro with considerable safety for mammalian cells. Antimicrobial mechanism is found to be changing osmotic pressure of cytoplasm in E. coli, causing cell deformation and destruction of S. aureus and C. albicans. In vivo anti-infection experiment demonstrated AIE PSs can accelerate the healing process of the burned wounds on rats infected by methicillin-resistant S. aureus (MRSA) or E. coli, indicating their potential to treat tertiary burns in clinical application. Therefore, the attained AIE PSs hold great promise as antimicrobial candidates in infective therapeutic application.(c) 2022 Elsevier Inc. All rights reserved.

Automated summary

This study successfully synthesized two efficient far-red/near-infrared photosensitizers with bright emission, which showed advantages in discriminating different types of bacteria and distinguishing dead and live cells, and demonstrated broad-spectrum antibacterial properties. The study also revealed the antimicrobial mechanism and showed the potential of these photosensitizers for treating infected burns in vivo.