4.6 Article

Salivary annexin A1: A candidate biomarker for periodontitis

Journal

JOURNAL OF CLINICAL PERIODONTOLOGY
Volume 50, Issue 7, Pages 942-951

Publisher

WILEY
DOI: 10.1111/jcpe.13803

Keywords

biomarker; clinical trial; molecular biology; periodontal diseases; saliva

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The aim of this study was to compare the salivary proteomic profile of periodontitis-affected parents and their offspring to periodontally healthy dyads in order to find potential biomarkers for early diagnosis of this disease. The results showed that ANXA1, KRT4, GSTP1, HPX, A2M, and KRT13 were lower in periodontitis-affected parents and their children, while IGHG1, CSTB, KRT9, SMR3B, IGHG4, and SERPINA1 were higher. ANXA1 had the lowest abundance and was selected as a potential biomarker for periodontitis. The in vitro assay also confirmed lower ANXA1 production in cells of periodontitis patients.
Aim To compare the salivary proteomic profile of periodontitis-affected (PA) parents and their offspring to periodontally healthy (PH) dyads in the pursuit of possible biomarkers for early diagnosis of this disease.Materials and Methods Unstimulated saliva samples collected from 17 pairs of PA or PH individuals and their children were submitted to mass spectrometric analyses followed by proteomic analyses. Primary PA fibroblasts were triggered towards having an inflammatory response, and an immunoenzymatic assay of its supernatant was performed to validate the obtained data.Results ANXA1, KRT4, GSTP1, HPX, A2M and KRT13 were lower in PA parents and their children, and IGHG1, CSTB, KRT9, SMR3B, IGHG4 and SERPINA1 were higher. ANXA1 presented the highest fold change, 7.1 times less produced in children of PA parents, and was selected as a potential biomarker for periodontitis. The in vitro assay also showed lower ANXA1 production by cells of PA patients.Conclusion Before any clinical sign of periodontal loss, descendants of PA patients have an altered proteomic profile compared to PH individuals, presenting a lower abundance of ANXA1. This protein is suggested as a potential biomarker for periodontitis.

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