4.5 Article

Dynein at the kinetochore

Related references

Note: Only part of the references are listed.
Article Cell Biology

Lis1-dynein drives corona compaction and limits erroneous microtubule attachment at kinetochores

Olivera Mitevska et al.

Summary: Mitotic cell division requires the formation of microtubule attachments for chromosome segregation and mitotic progression. The shedding of the fibrous corona, which is mediated by dynein, plays a crucial role in this process. Little is known about the regulation of dynein stripping and its response to attachment maturation.

JOURNAL OF CELL SCIENCE (2023)

Article Biochemistry & Molecular Biology

Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression

Jingchao Wu et al.

Summary: The fibrous corona, an outer-kinetochore meshwork structure, serves as an autonomous nucleation platform for kinetochore-derived microtubules. This nucleation relies on the LIC1 subunit of the dynein motor complex and the pericentrin protein. Fibrous coronas are important for spindle assembly, especially in acentrosomal cells like oocytes.

CURRENT BIOLOGY (2023)

Article Biochemistry & Molecular Biology

Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells

Fioranna Renda et al.

Summary: The proper separation of chromosomes during mitosis relies on load-bearing connections between sister kinetochores and opposite spindle poles via microtubule bundles. This study challenges the current models of spindle assembly by showing that most amphitelic attachments form synchronously at a specific stage and within a defined domain of the spindle. Structural and computational analyses suggest that interactions between kinetochores and microtubule bundles are mediated by noncentrosomal short microtubules.

CURRENT BIOLOGY (2022)

Article Biochemistry & Molecular Biology

Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores

Tobias Raisch et al.

Summary: A high-resolution cryo-EM structure of the ROD-Zwilch-ZW10 complex reveals essential features, such as a farnesyl-binding site required for Spindly binding. In vitro assays demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly complex, and the molecular mechanism of phosphorylation-dependent filament nucleation is described.

EMBO JOURNAL (2022)

Article Cell Biology

JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport

Ricardo Celestino et al.

Summary: The MAP kinase and motor scaffold JIP3 plays a role in preventing excessive accumulation of lysosomes in axons. The interaction between JIP3 and dynein and kinesin-1 is important for organelle clearance, but the specific mechanisms are not fully understood.

JOURNAL OF CELL BIOLOGY (2022)

Article Biology

Three-dimensional structure of kinetochore-fibers in human mitotic spindles

Robert Kiewisz et al.

Summary: In this study, the three-dimensional structure of kinetochore fibers in mammalian cells was characterized using large-scale electron tomography. It was found that each kinetochore fiber is composed of approximately nine kinetochore microtubules, with variations in circumference and density along their length. The association between kinetochore microtubules and non-kinetochore microtubules was primarily observed in the spindle pole regions.

ELIFE (2022)

Review Genetics & Heredity

The Four Causes: The Functional Architecture of Centromeres and Kinetochores

Andrew D. McAinsh et al.

Summary: This paper presents the components, assembly, structure, and function of kinetochores. The authors outline a blueprint for kinetochore assembly and discuss how functions are mapped onto this architecture. They also explore how the underlying chromatin influences kinetochore shape.

ANNUAL REVIEW OF GENETICS (2022)

Article Cell Biology

Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin

Ennio A. D'Amico et al.

Summary: The study provides a structure-function analysis of the Spindly protein, revealing its auto-inhibition mechanism and activation process. The researchers discovered that Spindly is activated by binding to two distinct kinetochore triggers, leading to conformational changes that unleash its Dynein-Dynactin adaptor activity. This study sheds light on the specific activation of Dynein at defined cellular locales.

JOURNAL OF CELL BIOLOGY (2022)

Article Multidisciplinary Sciences

Structure of dynein-dynactin on microtubules shows tandem adaptor binding

Sami Chaaban et al.

Summary: This study utilized cryo-electron microscopy processing to investigate the structure of the cytoplasmic dynein-dynactin complex and the adaptor BICDR1 bound to microtubules. The research revealed asymmetric interactions between neighboring dynein motor domains and provided insights into the contacts between adaptors and dynactin. This has implications for understanding the recruitment, stability, and stoichiometry of motor proteins.

NATURE (2022)

Article Cell Biology

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions

Amrita Kumari et al.

Summary: Mitotic phosphorylation of LIC1-CTD at its three cdk1 sites is essential for proper mitotic progression, dynein loading onto prometaphase kinetochores, and spindle assembly checkpoint inactivation in human cells. Phosphorylation also promotes the binding of Pin1 to Hook2dynein-Nde1-Lis1 complexes, affecting various aspects of mitotic processes.

JOURNAL OF CELL BIOLOGY (2021)

Article Biochemistry & Molecular Biology

The Disordered Spindly C-terminus Interacts with RZZ Subunits ROD-1 and ZWL-1 in the Kinetochore through the Same Sites in C. Elegans

Morkos A. Henen et al.

Summary: Spindly is a dynein adaptor that plays a role in chromosomal segregation during cell division. The C. elegans Spindly-C interacts with ROD-1 and Zwilch (ZWL-1) through specific binding sites, despite overall disorder in its structure. The presence of ZWL-1 may affect the interaction between Spindly-C and ROD-1 in the ROD-1/ZWL-1 complex context.

JOURNAL OF MOLECULAR BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end

Clinton K. Lau et al.

Summary: Dynactin is a complex that activates dynein for ultra-processive transport along microtubules. Its structure, formed by an actin-related filament, is defined by a flexible shoulder domain. By combining multiple cryo-EM datasets and precise masking strategies, the research overcame domain flexibility and obtained high-resolution maps of the Dynactin structure. The unique architecture of the shoulder securely positions subunits and reveals the molecular basis for cargo adaptor binding at the pointed end.

EMBO JOURNAL (2021)

Article Cell Biology

Dynein light intermediate chains as pivotal determinants of dynein multifunctionality

Amrita Kumari et al.

Summary: This study explores the mechanism of dynein in animal cells, specifically its role in microtubule transport by interacting with different cargoes. It suggests that the binding of dynein to adaptor proteins is tightly regulated, and different LIC subunits can perform unique and overlapping functions, with the C-terminal domains playing a crucial role in adaptor interaction. The authors hypothesize that various spatiotemporally regulated posttranslational modifications of LICs, as well as adaptors and cargoes, greatly expand the spectrum of dynein-adaptor-cargo complexes.

JOURNAL OF CELL SCIENCE (2021)

Article Multidisciplinary Sciences

On and off controls within dynein-dynactin on native cargoes

Paulomi Sanghavi et al.

Summary: Disrupting the interactions between dynactin and dynein or microtubules impacts dynein's behavior on microtubules and its ability to generate force. The dynactin-microtubule link is shown to be crucial for dynein's persistence against load, shedding light on dynein dysfunction.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Review Cell Biology

Role of spatial patterns and kinetochore architecture in spindle morphogenesis

Pablo Lara-Gonzalez et al.

Summary: The mitotic spindle is a macromolecular machine responsible for faithful chromosome segregation during cell division. Assembly of the spindle is guided by the 'Search & Capture' principle in structured space. Nucleation of noncentrosomal microtubules near kinetochores and changes in kinetochore architecture promote rapid and accurate assembly of the spindle.

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Polo regulates Spindly to prevent premature stabilization of kinetochore-microtubule attachments

Joao Barbosa et al.

EMBO JOURNAL (2020)

Review Biochemistry & Molecular Biology

Evolutionary Dynamics of the Spindle Assembly Checkpoint in Eukaryotes

Geert J. P. L. Kops et al.

CURRENT BIOLOGY (2020)

Article Cell Biology

The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance

Noopur V. Khobrekar et al.

DEVELOPMENTAL CELL (2020)

Article Biochemistry & Molecular Biology

Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint

Lindsey A. Allan et al.

EMBO JOURNAL (2020)

Review Cell Biology

Crowning the Kinetochore: The Fibrous Corona in Chromosome Segregation

Geert J. P. L. Kops et al.

TRENDS IN CELL BIOLOGY (2020)

Article Cell Biology

LIS1 promotes the formation of activated cytoplasmic dynein-1 complexes

Zaw Min Htet et al.

NATURE CELL BIOLOGY (2020)

Article Cell Biology

Lis1 activates dynein motility by modulating its pairing with dynactin

Mohamed M. Elshenawy et al.

NATURE CELL BIOLOGY (2020)

Article Multidisciplinary Sciences

A tunable LIC1-adaptor interaction modulates dynein activity in a cargo-specific manner

In-Gyun Lee et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex

Gang Zhang et al.

EMBO JOURNAL (2019)

Article Cell Biology

Spindle checkpoint silencing at kinetochores with submaximal microtubule occupancy

Banafsheh Etemad et al.

JOURNAL OF CELL SCIENCE (2019)

Article Multidisciplinary Sciences

Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions

Laura Pirovano et al.

NATURE COMMUNICATIONS (2019)

Article Cell Biology

Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner

Jonathan Kuhn et al.

JOURNAL OF CELL BIOLOGY (2019)

Review Cell Biology

Force-generating mechanisms of anaphase in human cells

Kruno Vukusic et al.

JOURNAL OF CELL SCIENCE (2019)

Article Multidisciplinary Sciences

Regulation of in vivo dynein force production by CDK5 and 14-3-3ε and KIAAO528

Dail E. Chapman et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases

Giuseppe Ciossani et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2018)

Article Cell Biology

Microtubules assemble near most kinetochores during early prometaphase in human cells

Vitali Sikirzhytski et al.

JOURNAL OF CELL BIOLOGY (2018)

Article Cell Biology

F-Actin nucleated on chromosomes coordinates their capture by microtubules in oocyte meiosis

Mariia Burdyniuk et al.

JOURNAL OF CELL BIOLOGY (2018)

Article Cell Biology

Cdk1 phosphorylation of the dynein adapter Nde1 controls cargo binding from G2 to anaphase

Caitlin L. Wynne et al.

JOURNAL OF CELL BIOLOGY (2018)

Article Cell Biology

The ESC RT protein Chmp4c regulates mitotic spindle checkpoint signaling

Eleni Petsalaki et al.

JOURNAL OF CELL BIOLOGY (2018)

Article Biochemistry & Molecular Biology

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

Danielle A. Grotjahn et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2018)

Article Biochemistry & Molecular Biology

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

Danielle A. Grotjahn et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2018)

Letter Biochemistry & Molecular Biology

Bub1 is not essential fn the checkpoint response to unattached kinetochores in diploid human cells

Cerys E. Currie et al.

CURRENT BIOLOGY (2018)

Article Multidisciplinary Sciences

Cryo-EM shows how dynactin recruits two dyneins for faster movement

Linas Urnavicius et al.

NATURE (2018)

Article Biochemistry & Molecular Biology

Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion

Jose-Antonio Rodriguez-Rodriguez et al.

CURRENT BIOLOGY (2018)

Article Cell Biology

Structure of the RZZ complex and molecular basis of its interaction with Spindly

Shyamal Mosalaganti et al.

JOURNAL OF CELL BIOLOGY (2017)

Article Cell Biology

Disease-associated mutations in human BICD2 hyperactivate motility of dynein-dynactin

Walter Huynh et al.

JOURNAL OF CELL BIOLOGY (2017)

Article Biology

NuMA recruits dynein activity to microtubule minus-ends at mitosis

Christina L. Hueschen et al.

ELIFE (2017)

Article Biochemistry & Molecular Biology

Evolutionary dynamics of the kinetochore network in eukaryotes as revealed by comparative genomics

Jolien J. E. van Hooff et al.

EMBO REPORTS (2017)

Article Biochemistry & Molecular Biology

Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated

Kai Zhang et al.

Review Biology

Mechanisms of Chromosome Congression during Mitosis

Helder Maiato et al.

BIOLOGY-BASEL (2017)

Article Biochemistry & Molecular Biology

Tyrosination of α-tubulin controls the initiation of processive dynein-dynactin motility

Richard J. McKenney et al.

EMBO JOURNAL (2016)

Article Cell Biology

Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities

Elise R. Pfaltzgraff et al.

MOLECULAR BIOLOGY OF THE CELL (2016)

Article Multidisciplinary Sciences

Load-induced enhancement of Dynein force production by LIS1-NudE in vivo and in vitro

Babu J. N. Reddy et al.

NATURE COMMUNICATIONS (2016)

Article Biochemistry & Molecular Biology

Structural basis for cargo binding and autoinhibition of Bicaudal-D1 by a parallel coiled-coil with homotypic registry

Shin-ichi Terawaki et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2015)

Article Biochemistry & Molecular Biology

RZZ and Mad1 dynamics in Drosophila mitosis

Lenaig Defachelles et al.

CHROMOSOME RESEARCH (2015)

Article Cell Biology

A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

Devinderjit K. Moudgil et al.

JOURNAL OF CELL BIOLOGY (2015)

Article Cell Biology

Adaptive changes in the kinetochore architecture facilitate proper spindle assembly

Valentin Magidson et al.

NATURE CELL BIOLOGY (2015)

Article Multidisciplinary Sciences

Microtubule detyrosination guides chromosomes during mitosis

Marin Barisic et al.

SCIENCE (2015)

Article Multidisciplinary Sciences

The structure of the dynactin complex and its interaction with dynein

Linas Urnavicius et al.

SCIENCE (2015)

Article Multidisciplinary Sciences

Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint

Gang Zhang et al.

NATURE COMMUNICATIONS (2015)

Article Biochemistry & Molecular Biology

In vitro reconstitution of a highly processive recombinant human dynein complex

Max A. Schlager et al.

EMBO JOURNAL (2014)

Article Biochemistry & Molecular Biology

Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles

Patricia M. A. Silva et al.

FEBS LETTERS (2014)

Article Biochemistry & Molecular Biology

LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complex

Hyang Mi Moon et al.

HUMAN MOLECULAR GENETICS (2014)

Article Cell Biology

Direct kinetochore-spindle pole connections are not required for chromosome segregation

Vitali Sikirzhytski et al.

JOURNAL OF CELL BIOLOGY (2014)

Article Cell Biology

Force on spindle microtubule minus ends moves chromosomes

Mary Williard Elting et al.

JOURNAL OF CELL BIOLOGY (2014)

Article Multidisciplinary Sciences

Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes

Richard J. McKenney et al.

SCIENCE (2014)

Article Biochemistry & Molecular Biology

Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit

Ting-Yu Yeh et al.

EMBO JOURNAL (2013)

Article Cell Biology

Systematic dissection of dynein regulators in mitosis

Jonne A. Raaijmakers et al.

JOURNAL OF CELL BIOLOGY (2013)

Article Cell Biology

Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis

Shusheng Wang et al.

MOLECULAR BIOLOGY OF THE CELL (2013)

Article Multidisciplinary Sciences

Crosstalk Between Microtubule Attachment Complexes Ensures Accurate Chromosome Segregation

Dhanya K. Cheerambathur et al.

SCIENCE (2013)

Article Biochemistry & Molecular Biology

The Spatial Arrangement of Chromosomes during Prometaphase Facilitates Spindle Assembly

Valentin Magidson et al.

Article Biochemistry & Molecular Biology

Identification of a Novel Dynein Binding Domain in Nudel Essential for Spindle Pole Organization in Xenopus Egg Extract

Shusheng Wang et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Polo-like Kinase1 Is Required for Recruitment of Dynein to Kinetochores during Mitosis

Jason R. Bader et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Mutually Exclusive Cytoplasmic Dynein Regulation by NudE-Lis1 and Dynactin

Richard J. McKenney et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Cell Biology

The N-terminal coiled-coil of Ndell is a regulated scaffold that recruits LIS1 to dynein

Eliza Zylkiewicz et al.

JOURNAL OF CELL BIOLOGY (2011)

Article Cell Biology

A Nup133-dependent NPC-anchored network tethers centrosomes to the nuclear envelope in prophase

Stephanie Bolhy et al.

JOURNAL OF CELL BIOLOGY (2011)

Article Cell Biology

Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on kinetochores

James M. Kasuboski et al.

MOLECULAR BIOLOGY OF THE CELL (2011)

Article Biochemistry & Molecular Biology

LIS1 and NudE Induce a Persistent Dynein Force-Producing State

Richard J. McKenney et al.

Article Cell Biology

Spindly/CCDC99 Is Required for Efficient Chromosome Congression and Mitotic Checkpoint Regulation

Marin Barisic et al.

MOLECULAR BIOLOGY OF THE CELL (2010)

Article Biochemistry & Molecular Biology

Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint

Mylavarapu V. S. Sivaram et al.

EMBO JOURNAL (2009)

Article Cell Biology

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly

Ying Wai Chan et al.

JOURNAL OF CELL BIOLOGY (2009)

Article Cell Biology

Roles of the Drosophila NudE protein in kinetochore function and centrosome migration

Alan Wainman et al.

JOURNAL OF CELL SCIENCE (2009)

Article Cell Biology

Chromosome congression in the absence of kinetochore fibres

Shang Cai et al.

NATURE CELL BIOLOGY (2009)

Article Cell Biology

Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis

Jacqueline Whyte et al.

JOURNAL OF CELL BIOLOGY (2008)

Article Biochemistry & Molecular Biology

Cenp-F links kinetochores to Ndel1/Nde1/Lis1/Dynein microtubule motor complexes

Mailys A. S. Vergnolle et al.

CURRENT BIOLOGY (2007)

Article Cell Biology

Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli

Yun Liang et al.

MOLECULAR BIOLOGY OF THE CELL (2007)

Article Biochemistry & Molecular Biology

Kinetochore dynein is required for chromosome motion and congression independent of the spindle checkpoint

Zhenye Yang et al.

CURRENT BIOLOGY (2007)

Article Cell Biology

Mitosin/CENP-F in mitosis, transcriptional control, and differentiation

L Ma et al.

JOURNAL OF BIOMEDICAL SCIENCE (2006)

Article Multidisciplinary Sciences

Chromosomes can congress to the metaphase plate before biorientation

TM Kapoor et al.

SCIENCE (2006)

Article Biochemistry & Molecular Biology

Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F

P Bomont et al.

EMBO JOURNAL (2005)

Article Multidisciplinary Sciences

Cytoplasmic LEM is a regulator of microtubule function through its interaction with the LIS1 pathway

V Soukoulis et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)

Article Biochemistry & Molecular Biology

Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex

E Buffin et al.

CURRENT BIOLOGY (2005)

Article Cell Biology

ZW10 links mitotic checkpoint signaling to the structural kinetochore

GJPL Kops et al.

JOURNAL OF CELL BIOLOGY (2005)

Article Biochemistry & Molecular Biology

In vivo dynamics of the rough deal checkpoint protein during Drosophila mitosis

R Basto et al.

CURRENT BIOLOGY (2004)

Article Biochemistry & Molecular Biology

Spindle checkpoint protein dynamics at kinetochores in living cells

BJ Howell et al.

CURRENT BIOLOGY (2004)

Article Biochemistry & Molecular Biology

Bicaudal D induces selective dynein-mediated microtubule minus end-directed transport

CC Hoogenraad et al.

EMBO JOURNAL (2003)

Article Cell Biology

Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions

BC Williams et al.

MOLECULAR BIOLOGY OF THE CELL (2003)

Article Cell Biology

Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

A Khodjakov et al.

JOURNAL OF CELL BIOLOGY (2003)

Article Biochemistry & Molecular Biology

Human nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle

XM Yan et al.

MOLECULAR AND CELLULAR BIOLOGY (2003)

Article Biochemistry & Molecular Biology

Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin

SH Tynan et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2000)