Journal
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Volume 149, Issue 11, Pages 8711-8718Publisher
SPRINGER
DOI: 10.1007/s00432-023-04822-y
Keywords
Head and neck squamous cell carcinoma; Pillar[5]arene; Apoptosis; Caspase
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This study demonstrates that the newly synthesized derivatives of pillar[5]arene, d-Q-P5 and p-Q-P5, effectively inhibit the proliferation of Squamous cell carcinomas of the head and neck (HNSCC) cells by upregulating proapoptotic and caspase pathway genes, while downregulating antiapoptotic gene.
PurposeThis study was conducted to investigate impacts of potential anticancer (associated with apoptosis and caspase pathways) of two newly synthesized derivatives of pillar[5]arene, named as d-Q-P5 and p-Q-P5, on Squamous cell carcinomas of the head and neck (HNSCC) cells.Materials and methodsThe MTT method was used to determine the IC50 doses of the derivatives on HNSCC cells, and the changes in gene expression were analyzed by real-time polymerase chain reaction (qPCR). The apoptosis change was confirmed by flow cytometry analysis.ResultsThe results showed that the d-Q-P5 and p-Q-P5 effectively inhibited the proliferation of the cells by upregulating proapoptotic genes (Bax, Bad, p53, Bak, and Apaf-1) and genes involved in the caspase pathway (Casp2, Casp3, and Casp9), while downregulating the antiapoptotic gene (Bcl-2).ConclusionsThis study is the first to demonstrate the potential anticancer effects of these two agents on HNSCC cells by positively regulating apoptosis gene expression.
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