Conservation, abundance, glycosylation profile, and localization of the TSP protein family in Cryptosporidium parvum

Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling cryptosporidiosis. At present, the only approved vaccine against any apicomplexan parasite targets a conserved adhesin possessing a thrombospondin repeat domain. C. parvum possesses 12 orthologous thrombospondin repeat domain-containing proteins known as CpTSP1-12, though little is known about these potentially important antigens. Here, we explore the architecture and conservation of the CpTSP protein family, as well as their abundance at the protein level within the sporozoite stage of the life cycle. We examine the glycosylation states of these proteins using a combination of glycopeptide enrichment techniques to demonstrate that these proteins are modified with C-, O-, and N-linked glycans. Using expansion microscopy, and an antibody against the C-linked mannose that is unique to the CpTSP protein family within C. parvum, we show that these proteins are found both on the cell surface and in structures that resemble the secretory pathway of C. parvum sporozoites. Finally, we generated a polyclonal antibody against CpTSP1 to show that it is found at the cell surface and within micronemes, in a pattern reminiscent of other apicomplexan motility-associated adhesins, and is present both in sporozoites and meronts. This work sheds new light on an undervaccines against cryptosporidiosis.

Automated summary

Cryptosporidium parvum is a common cause of diarrheal disease worldwide and developing vaccines to prevent or limit infection is important. The study investigates the architecture, conservation, and glycosylation of the CpTSP protein family in C. parvum. The results show that these proteins are modified with C-, O-, and N-linked glycans and are found on the cell surface and in secretory pathway-like structures. The study also reveals the presence of CpTSP1 in sporozoites and meronts, shedding new light on potential vaccine targets for cryptosporidiosis.