Trans-Hydroxy, Trans-Epoxy, and Erythro-dihydroxy Fatty Acids Increase during Deep-Frying

Article Biochemical Research Methods

Development of a quantitative proteomics approach for cyclooxygenases and lipoxygenases in parallel to quantitative oxylipin analysis allowing the comprehensive investigation of the arachidonic acid cascade

Nicole M. Hartung et al.

Summary: This study describes a quantitative multi-omics approach combining liquid chromatography tandem mass spectrometry-based targeted oxylipin metabolomics and proteomics. It enables the simultaneous quantitative analysis of human COX and LOX pathway enzymes and 239 oxylipins. Using this sensitive multi-omics approach, the researchers comprehensively characterized oxylipin and protein patterns in human monocytic cell line THP-1 and differently polarized primary macrophages, demonstrating its usefulness in studying molecular modes of action involved in the modulation of the ARA cascade.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2023)

Add to Collection

Article Biochemical Research Methods

Rapid quantification of fatty acids in plant oils and biological samples by LC-MS

Elisabeth Koch et al.

Summary: This study developed a rapid and sensitive LC-MS method for quantification of saturated and unsaturated fatty acids, demonstrating high accuracy and precision in analysis. The method was successfully applied on human plasma and edible oils, showing potential for gaining new insights in the composition of biological samples.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Food Science & Technology

Effects of Potato Processing and Frying on Oxylipin Concentrations

Zhichao Zhang et al.

Summary: This study comprehensively measured the effects of potato processing on the concentration of oxidized fatty acid products known as oxylipins, finding that distinct oxylipin profiles are observed when oil and potatoes are subjected to different processing conditions.

ACS FOOD SCIENCE & TECHNOLOGY (2021)

Add to Collection

Article Biochemistry & Molecular Biology