4.7 Article

Production of Four Flavonoid C-Glucosides in Escherichia coli

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 71, Issue 13, Pages 5302-5313

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.3c00297

Keywords

C-glucosyltransferase; biosynthesis; Escherichia coli; apigenin C-glucoside; kaempferol C-glucoside; metabolic engineering

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In this study, a pathway was introduced to Escherichia coli to synthesize four flavonoid C-glucosides. The synthesis pathway involved the use of two regioselective flavonoid C-glycosyltransferases and resulted in the synthesis of isovitexin, vitexin, kaempferol 6-C-glucoside, and kaempferol 8-C-glucoside. The synthesized flavonoid C-glucosides provide a basis for investigating their novel biological properties.
Flavonoid C-glucosides, which are found in several plant families, are characterized by several biological properties, including antioxidant, anticancer, anti-inflammatory, neuroprotective, hepatoprotective, cardioprotective, antibacterial, antihyperalgesic, antiviral, and antinociceptive activities. The biosynthetic pathway of flavonoid C-glucosides in plants has been elucidated. In the present study, a pathway was introduced to Escherichia coli to synthesize four flavonoid C-glucosides, namely, isovitexin, vitexin, kaempferol 6-C-glucoside, and kaempferol 8-C-glucoside. A five- or six-step metabolic pathway for synthesizing flavonoid aglycones from tyrosine was constructed and two regioselective flavonoid C-glycosyltransferases from Wasabia japonica (WjGT1) and Trollius chinensis (TcCGT) were used. Additionally, the best shikimate gene module construct was selected to maximize the titer of each Cglucoside flavonoid. Isovitexin (30.2 mg/L), vitexin (93.9 mg/L), kaempferol 6-C-glucoside (14.4 mg/L), and kaempferol 8-Cglucoside (38.6 mg/L) were synthesized using these approaches. The flavonoid C-glucosides synthesized in this study provide a basis for investigating and unraveling their novel biological properties.

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