Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus

To cope with the easy transmissibility of the avian influenza A virus subtype H1N1, a biosensor was developed for rapid and highly sensitive electrochemical immunoassay. Based on the principle of specific binding between antibody and virus molecules, the active molecule-antibody-adapter structure was formed on the surface of an Au NP substrate electrode; it included a highly specific surface area and good electrochemical activity for selective amplification detection of the H1N1 virus. The electrochemical test results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode was used for the electrochemical detection of the H1N1 virus with a sensitivity of 92.1 mu A (pg/mL)(-1) cm(2), LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of (R-2 = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for the molecular detection of the H1N1 virus will be of great use in the field of epidemic prevention and raw poultry protection.

Automated summary

To combat the highly transmissible avian influenza A virus subtype H1N1, a biosensor was developed for rapid and sensitive electrochemical immunoassay. By employing antibody-virus binding, an active molecule-antibody-adapter structure was created on an Au NP substrate electrode, providing a specific surface area and good electrochemical activity for selective amplification detection of H1N1 virus. The results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode achieved a sensitivity of 92.1 muA (pg/mL)(-1) cm(2), LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of (R-2 = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for molecular detection of the H1N1 virus will be highly valuable in epidemic prevention and raw poultry protection.