Yinghuangia soli sp. nov., isolated from soil of weathering dolomite in a karst area

A Gram- stain-positive, aerobic actinomycete strain, designated KLBMP 8922T, was isolated from a soil sample collected from weathering dolomite crust in Guizhou Province, PR China. KLBMP 8922T showed the 16S rRNA gene similarities to Yinghuangia seranimata CCTCC AA 206006T (98.7 %), Yinghuangia catbensis VN07A0015T (98.3 %) and Yinghuangia aomiensis M24DS4T (98.2 %). The taxonomic status of this strain was investigated by using a polyphasic approach. The aerial mycelia of KLBMP 8922T formed spore chains, and spores were cylindrical with smooth surfaces. The whole -cell sugars were ribose, mannose and galactose with traces of glucose and xylose. The diagnostic amino acids of the cell wall were ll- diaminopimelic acid, alanine and glutamic acid. The predominant menaquinones were MK -9(H6) and MK-9(H8). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylethanolamine, an unidentified phospholipid and an uniden-tified lipid. The major cellular fatty acids (>10 %) were iso-C15:0, iso-C16: 0 and iso-C16:1H. The genomic DNA G+C content was 72.0 mol%. The digital DNA-DNA hybridization (dDDH) value between KLBMP 8922T and Y. seranimata CCTCC AA 206006T was 24.1 %, and the average nucleotide identity (ANI) value was 81.0 %. On the basis of a combination of morphological, chemotaxo-nomic and phylogenetic characteristics, strain KLBMP 8922T represents a novel species of the genus Yinghuangia for which the name Yinghuangia soli sp. nov. is proposed. The type strain was KLBMP 8922T (= CGMCC 1.19360T = NBRC 115572T).

Automated summary

A new Gram-stain-positive, aerobic actinomycete strain named KLBMP 8922T was isolated from a soil sample in Guizhou Province, China. Through a polyphasic approach, it was confirmed that KLBMP 8922T represents a novel species of the genus Yinghuangia, and the name Yinghuangia soli sp. nov. is proposed.