Vibrio intestinalis sp. nov., isolated from intestine of seahorse

A novel Gram- stain- negative, catalase- and oxidase- positive, facultatively anaerobic, and rod- shaped motile bacterial strain, designated as YLB- 11T, was isolated from seahorse intestine. The 16S rRNA gene sequencing analysis showed that YLB-11T was most closely related Vibrio mytili LMG 19157T (98.9 % nucleotide sequence identity). Phylogenetic analysis placed strain YLB-11T within the genus Vibrio. The major cellular fatty acids were summed feature 3 (C16: 1 w6c/C16 :1 w7c, 36.4%), C16: 0 (19.1 %) and summed feature 8 (C18:1 w6c/C18:1 w7c, 12.3 %). The DNA G+C content of YLB- 11T was 44.7 mol %. The in silico DNA-DNA hybridization and average nucleotide identity values for whole- genome sequence comparisons between YLB-11T and related species were clearly below the thresholds used for the delineation of a novel species. Therefore, YLB-11T is considered to represent novel species of the genus Vibrio, for which the name Vibrio intestinalis sp. nov. is proposed. The type strain is YLB- 11T (=MCCC 1A17441T=KCTC 72604T).

Automated summary

A novel Gram-stain-negative, facultatively anaerobic and rod-shaped motile bacterium, designated as YLB-11T, was isolated from seahorse intestine. It shows the closest relationship with Vibrio mytili LMG 19157T (98.9% nucleotide sequence identity) based on 16S rRNA gene sequencing analysis. Phylogenetic analysis places it within the genus Vibrio. The major cellular fatty acids include summed feature 3 (C16:1 w6c/C16 :1 w7c, 36.4%), C16:0 (19.1%), and summed feature 8 (C18:1 w6c/C18:1 w7c, 12.3%).