Radiobacillus kanasensis sp. nov., a halotolerant bacterium isolated from woodland soil

A novel Gram-positive, aerobic, rod-shaped, non-motile, endospore-forming salt-tolerant bacterium strain (80(T)), was isolated from woodland soil collected near Kanas lake in the Altay region of Xinjiang, PR China. The strain grew at 15-45 degrees C, pH6.0-9.0 and with 0-14% (w/v) NaCl. The complete genome size of the novel strain was 4031 766 bp including a circle chromosome and a circle plasmid. The genomic DNA G+C content was 38.99 mol %. Phylogenetic analysis based on 16S rRNA gene sequence and genome showed that strain 80(T) has the highest similarity to Radiobacillus deserti TKL69(T). However, the novel strain showed an average nucleotide identity value of 78.65% (lower than 95 %) and a digital DNA-DNA hybridization value of 22.30% with R. deserti TKL69(T) based on the genome sequences. The major fatty acids were anteiso-C 15 :0, iso-C (15 :0), anteiso-C (17:0) and C (16:1) omega 7c alcohol. The only respiratory quinone was MK-7. The cell wall peptidoglycan was meso-diaminopimelic acid. Diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified glycolipids were identified as the major polar lipids. The phylogenetic, phenotypic and chemotaxonomic analyses showed that strain 80T represents a novel species of the genus Radiobacillus and the name Radiobacillus kanasensis sp. nov. is proposed. The type strain is 80(T) (=GDMCC 1.2844(T)=JCM 35077(T)).

Automated summary

A new strain of gram-positive, aerobic, rod-shaped, non-motile, endospore-forming bacterium (80(T)) with salt tolerance was isolated from woodland soil near Kanas lake in the Altay region of Xinjiang, PR China. The strain grew under a wide range of temperature, pH, and NaCl concentration. The complete genome of the strain was sequenced and analyzed, revealing its distinctness from known strains. The strain represents a novel species within the genus Radiobacillus, named Radiobacillus kanasensis sp. nov.