Collibacillus ludicampi gen. nov., sp. nov., a new soil bacterium of the family Alicyclobacillaceae

A novel moderately thermophilic aerobic bacterium, strain TP075T, was isolated from soil collected from an athletic field in Japan. Strain TP075T is a rod- shaped, aerobic bacterium that forms terminal endospores. The KOH lysis test suggested that the cell wall of the isolate has a Gram- positive structure. For aerobic growth, the optimum pH and temperature were 4.0-5.0 and 47-50 degrees C, respectively. Draft genome sequencing showed that the G+C content of genomic DNA was 46.5 mol%. Branched- chain fatty acids (iso- C15:0, anteiso-C15: 0 and iso- C16:0) were the major components of the cellular fatty acid profile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain TP075T belongs to the family Alicyclobacillaceae, with the highest similarity to Effusibacillus consociatus CCUG53762T (92.6%) and Tumebacillus soil CAU11108T (92.5%). Genome- based analyses indicated that strain TP075T and the most closely related strain, Effusibacillus pohliae DSM 22757T, share an average amino acid identity value of 62.57% and an average nucleotide identity value of 70.86 %. The results obtained in this study suggest that strain TP075T represents a novel species of a novel genus, for which we propose the name Collibacillus ludicampi gen. nov., sp. nov. with type strain TP075T (= JCM 34430T=TBRC 15189T).

Automated summary

A novel moderately thermophilic aerobic bacterium, Collibacillus ludicampi, was isolated from soil in Japan. It belongs to the family Alicyclobacillaceae and shows highest similarity to Effusibacillus consociatus and Tumebacillus soil. The strain represents a novel species of a novel genus.