Dyadobacter pollutisoli sp. nov., isolated from plastic waste landfill soil

A Gram- stain-negative, yellow-pigmented and facultatively aerobic bacterium, designated strain U1(T), was isolated from plastic dumped soil sampled in the Republic of Korea. Cell of strain U1(T) were non-motile rods showing catalase-negative and oxidase-positive activities. Strain U1T was shown to grow at 10-37 degrees C (optimum, 25-30 degrees C) and pH 6.0-9.0 (optimum, pH 8.0), and in the presence of 0-0.5 % (w/v) NaCl (optimum, 0 %). Strain U1T contained iso-C(15:)0, C-16:0,C-16:1 omega 5c and summed feature 3 (comprising C-16:1 omega 6c and/or C(16:)1 omega 7c) as the major cellular fatty acids (>5 %) and menaquinone- 7 as the sole respiratory quinone. Phos-phatidylethanolamine, two unidentified aminolipids and three unidentified lipids were identified as the major polar lipids. The DNA G+C content of strain U1(T) calculated from the whole-genome sequence was 45.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain U1(T) formed a distinct phylogenetic lineage within the genus Dyadobacter. Strain U1(T) has the highest 16S rRNA sequence similarity to Dyadobacter bucti QTA69(T) (97.9 %). Average nucleotide identity and digital DNA-DNA hybridization values between strain U1(T) and D. bucti QTA69(T) were 74.6 % and 18.9 %, respectively. Based on pheno-typic, chemotaxonomic and molecular features, strain U1(T) represents a novel species of the genus Dyadobacter, for which the name Dyadobacter pollutisoli sp. nov. is proposed. The type strain is U1(T) (= KACC 22210T=JCM 34491(T)).

Automated summary

A Gram-stain-negative, yellow-pigmented and facultatively aerobic bacterium, strain U1(T), was isolated from plastic dumped soil in Korea. The strain showed specific characteristics, such as non-motile rods, catalase-negative and oxidase-positive activities. Phylogenetic analysis confirmed that strain U1(T) belongs to a new species of the genus Dyadobacter.