Antidiabetic potential of Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) on Streptozotocin-induced diabetic rats-An implication on in vivo approach

Gymnemic acid is glycosides of triterpene with recognized and valuable applications for several chronic diseases, mainly diabetics. Despite this, it requires a delivery system in order to range its therapeutic target due to its limited solubility and bioavailability. Therefore, the Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) was synthesised by eco-friendly approach. The synthesised Gym@AuNPs was confirmed by the colour change from light yellow to a deep ruby red. UV -visible spectroscopy results showed a strong narrow peak at 530 nm, confirming the controlled synthesis of monodispersed Gym@AuNPs. The reduction potential of standard Gym-nemic acid (Gym) on synthesis of Gym@AuNPs was confirmed by using HPLC analysis. The spherical shaped Gym@AuNPs was observed by FESEM and HR-TEM studies with average size of 48.52 +/- 5.53 nm. The XRD analysis exhibited a face-centered cubic (FCC) crystalline nature of Gym@AuNPs. The in vivo antidiabetic activity of Gym and Gym@AuNPs were validated using Streptozotocin induced diabetic Albino wistar rats. The Gym@AuNPs and Gym were regulates the glucose and lipid levels in experimental animals. The histopathology outcomes shown that the Gym@AuNPs were restoration of pancreatic islets cells in the animals. This investi-gation demonstrated that the Gym@AuNPs had the potential anti-diabetic properties.

Automated summary

Gymnemic acid, a glycoside of triterpene, has valuable applications for chronic diseases, particularly for diabetes. However, its limited solubility and bioavailability necessitate the use of a delivery system. In this study, Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) were synthesized using an eco-friendly approach. The synthesized Gym@AuNPs exhibited a deep ruby red color, a narrow peak at 530 nm on UV-visible spectroscopy, and a spherical shape with an average size of 48.52 +/- 5.53 nm on FESEM and HR-TEM studies. The in vivo antidiabetic activity of Gym@AuNPs was validated in diabetic rats, showing regulation of glucose and lipid levels as well as restoration of pancreatic islet cells.