4.7 Article

The Expression of Autophagy-Associated Genes Represents a Valid Footprint for Aggressive Pancreatic Neuroendocrine Neoplasms

Journal

Publisher

MDPI
DOI: 10.3390/ijms24043636

Keywords

pancreatic neuroendocrine neoplasm; pancreatic neuroendocrine tumor; cancer; MEN1; autophagy; mTOR; beclin1

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This study aims to investigate the association between the expression of autophagy-associated gene transcripts and clinical parameters in pNEN. The results show that G1 sporadic pNEN have higher expression of autophagic genes compared to G2, and pNEN with decreased expression of autophagic genes are more likely to have lymphatic and distant metastasis. Insulinomas have higher levels of autophagic transcripts compared to gastrinomas and non-functional pNEN. MEN1-associated pNEN exhibit higher expression of autophagic genes than sporadic pNEN.
Pancreatic neuroendocrine neoplasms (pNEN) are rare and heterogeneous tumors. Previous investigations have shown that autophagy can be a target for cancer therapy. This study aimed to determine the association between the expression of autophagy-associated gene transcripts and clinical parameters in pNEN. In total, 54 pNEN specimens were obtained from our human biobank. The patient characteristics were retrieved from the medical record. RT-qPCR was performed to assess the expression of the autophagic transcripts BECN1, MAP1LC3B, SQSTM1, UVRAG, TFEB, PRKAA1, and PRKAA2 in the pNEN specimens. A Mann-Whitney U test was used to detect differences in the expression of autophagic gene transcripts between different tumor characteristics. This study showed that G1 sporadic pNEN have a higher expression of autophagic genes compared to G2. Lymphatic and distant metastasis occurred significantly more often in pNEN with a decreased expression of the autophagic genes. Within sporadic pNEN, the insulinomas express higher levels of autophagic transcripts than gastrinomas and non-functional pNEN. MEN1-associated pNEN show a higher expression of autophagic genes than sporadic pNEN. In summary, a decreased expression of autophagic transcripts distinguishes metastatic from non-metastatic sporadic pNEN. The significance of autophagy as a molecular marker for prognosis and therapy decisions needs to be further investigated.

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