Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/ijms24043659
Keywords
hemoglobin; heme-iron; silica nanoparticles; adsorption; XAS; visible circular dichroism
Ask authors/readers for more resources
The adsorption of proteins on surfaces and its relationship with the structural and functional properties of the adsorbed protein and the adsorption mechanism were studied using hemoglobin adsorbed on silica nanoparticles. It was found that hemoglobin's affinity towards oxygen increased with adsorption, but there were no significant changes in the quaternary and secondary structures. The focus of this work was on the active sites of hemoglobin, the heme and its iron, and it was found that the modifications in the heme pocket environment due to changes in the angles of the heme vinyl functions could explain the greater affinity observed.
The adsorption of proteins on surfaces has been studied for a long time, but the relationship between the structural and functional properties of the adsorbed protein and the adsorption mechanism remains unclear. Using hemoglobin adsorbed on silica nanoparticles, we have previously shown that hemoglobin's affinity towards oxygen increases with adsorption. Nevertheless, it was also shown that there were no significant changes in the quaternary and secondary structures. In order to understand the change in activity, we decided in this work to focus on the active sites of hemoglobin, the heme and its iron. After measuring adsorption isotherms of porcine hemoglobin on Ludox silica nanoparticles, we analyzed the structural modifications of adsorbed hemoglobin by X-ray absorption spectroscopy and circular dichroism spectra in the Soret region. It was found that upon adsorption, there were modifications in the heme pocket environment due to changes in the angles of the heme vinyl functions. These alterations can explain the greater affinity observed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available