A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device

According to the World Health Organization (WHO) forecasts, Antimicrobial Resistance (AMR) will be the leading cause of death worldwide in the next decades. To prevent this phenomenon, rapid Antimicrobial Susceptibility Testing (AST) techniques are required to drive the selection of the most suitable antibiotic and its dosage. In this context, we propose an on-chip platform, based on a micromixer and a microfluidic channel, combined with a pattern of engineered electrodes to exploit the di-electrophoresis (DEP) effect. The role of the micromixer is to ensure the proper interaction of the antibiotic with the bacteria over a long time (approximate to 1 h), and the DEP-based microfluidic channel enables the efficient sorting of live from dead bacteria. A sorting efficiency of more than 98%, with low power consumption (V-pp = 1 V) and time response of 5 s, within a chip footprint of approximate to 86 mm(2), has been calculated, which makes the proposed system very attractive and innovative for efficient and rapid monitoring of the antimicrobial susceptibility at the single-bacterium level in next-generation medicine.

Automated summary

According to the World Health Organization (WHO) forecasts, Antimicrobial Resistance (AMR) will be the leading cause of death worldwide in the next decades. To address this issue, a new on-chip platform based on micromixers and microfluidic channels with engineered electrodes has been proposed. This platform utilizes di-electrophoresis (DEP) effect to efficiently sort live and dead bacteria, enabling rapid monitoring of antimicrobial susceptibility at the single-bacterium level. The system shows high sorting efficiency, low power consumption, and fast response time, making it an attractive and innovative solution for next-generation medicine.