Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ijms24087246
Keywords
electromagnetic pulse (EMP); reactive oxygen species (ROS); human mesenchymal stem cells (hMSC); electron paramagnetic resonance (EPR); scanning electron microscope (SEM); Marx generator
Ask authors/readers for more resources
The research on the effect of nanosecond electromagnetic pulses on human health, especially the formation of free radicals in human cells, is ongoing. This study conducted a preliminary investigation on the impact of a single high-energy electromagnetic pulse on morphology, viability, and free radical generation in human mesenchymal stem cells (hMSC). The results showed that the exposure to the high-energy electromagnetic pulse did not affect the number of free radicals generated or the morphology of hMSC in vitro compared to control samples, as observed through microscopic examination and EPR measurements.
The effect of nanosecond electromagnetic pulses on human health, and especially on forming free radicals in human cells, is the subject of continuous research and ongoing discussion. This work presents a preliminary study on the effect of a single high-energy electromagnetic pulse on morphology, viability, and free radical generation in human mesenchymal stem cells (hMSC). The cells were exposed to a single electromagnetic pulse with an electric field magnitude of similar to 1 MV/m and a pulse duration of similar to 120 ns generated from a 600 kV Marx generator. The cell viability and morphology at 2 h and 24 h after exposure were examined using confocal fluorescent microscopy and scanning electron microscopy (SEM), respectively. The number of free radicals was investigated with electron paramagnetic resonance (EPR). The microscopic observations and EPR measurements showed that the exposure to the high-energy electromagnetic pulse influenced neither the number of free radicals generated nor the morphology of hMSC in vitro compared to control samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available