4.7 Article

Sleep Fragmentation Accelerates Carcinogenesis in a Chemical-Induced Colon Cancer Model

Journal

Publisher

MDPI
DOI: 10.3390/ijms24054547

Keywords

carcinogenesis; colon cancer; reactive oxygen species; sleep fragmentation

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The study aimed to investigate the impact of sleep fragmentation on carcinogenesis and explore the underlying mechanisms in a chemical-induced colon cancer model. The results showed that mice subjected to sleep fragmentation had a significantly higher number and size of tumors compared to the control group. Immunohistochemical and immunofluorescent stainings revealed increased DNA damage and oxidative stress in the sleep fragmentation group.
Aims of this study were to test whether sleep fragmentation (SF) increased carcinogenesis and to investigate the possible mechanisms of carcinogenesis in a chemical-induced colon cancer model. In this study, eight-week-old C57BL/6 mice were divided into Home cage (HC) and SF groups. After the azoxymethane (AOM) injection, the mice in the SF group were subjected to SF for 77 days. SF was accomplished in a sleep fragmentation chamber. In the second protocol, mice were divided into 2% dextran sodium sulfate (DSS)-treated, HC, and SF groups and were exposed to the HC or SF procedures. Immunohistochemical and immunofluorescent stainings were conducted to determine the level of 8-OHdG and reactive oxygen species (ROS), respectively. Quantitative real-time polymerase chain reaction was used to assess the relative expression of inflammatory and ROS-generating genes. The number of tumors and average tumor size were significantly higher in the SF group than in the HC group. The intensity (%) of the 8-OHdG stained area was significantly higher in the SF group than in the HC group. The fluorescence intensity of ROS was significantly higher in the SF group than the HC group. SF accelerated cancer development in a murine AOM/DSS-induced model of colon cancer, and the increased carcinogenesis was associated with ROS- and oxidative stress-induced DNA damage.

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