Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/ijms24108674
Keywords
anellovirus; quantitative PCR; next-generation sequencing; primer set; quality score
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This study aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR method in identifying the etiology of KD. The results showed that the TTV loads in whole blood and nasopharyngeal aspirates were highly correlated, supporting the validity of the ssTTV-PCR method. However, inconsistencies were observed when the ssTTV-PCR was more sensitive than NGS or when there were mismatches between the primer sequences and viral sequences.
A next-generation sequencing (NGS) study identified a very high viral load of Torquetenovirus (TTV) in KD patients. We aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR (ssTTV-PCR) method to identify the etiology of KD. We applied ssTTV-PCR to samples collected from 11 KD patients and 22 matched control subjects who participated in our previous prospective study. We used the NGS dataset from the previous study to validate ssTTV-PCR. The TTV loads in whole blood and nasopharyngeal aspirates correlated highly (Spearman's R = 0.8931, p < 0.0001, n = 33), supporting the validity of ssTTV-PCR. The ssTTV-PCR and NGS results were largely consistent. However, inconsistencies occurred when ssTTV-PCR was more sensitive than NGS, when the PCR primer sequences mismatched the viral sequences in the participants, and when the NGS quality score was low. Interpretation of NGS requires complex procedures. ssTTV-PCR is more sensitive than NGS but may fail to detect a fast-evolving TTV species. It would be prudent to update primer sets using NGS data. With this precaution, ssTTV-PCR can be used reliably in a future large-scale etiological study for KD.
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