Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/ijms24097805
Keywords
marine toxins; C17-SAMT; in vitro; micronucleus assay; oxidative stress; mitochondrial dysfunction; gamma H2AX; pH3 phospho S10
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This study investigated the genotoxicity and cytotoxicity of C17-sphinganine analog mycotoxin (C17-SAMT) through in vitro assays. The results showed that C17-SAMT induced an increase in micronucleus formation in human lymphoblastoid TK6 cells and mitochondrial dysfunction in HepaRG cells. Further research is needed to fully understand the mode of action of this toxin. The study highlights the potential toxicity of C17-SAMT and emphasizes the importance of further research.
This study investigates the genotoxicity and cytotoxicity of C17-sphinganine analog mycotoxin (C17-SAMT) using in vitro assays. C17-SAMT was previously identified as the cause of unusual toxicity in cultured mussels from the Bizerte Lagoon in northern Tunisia. While a previous in vivo genotoxicity study was inconclusive, in vitro results demonstrated that C17-SAMT induced an increase in micronucleus formation in human lymphoblastoid TK6 cells at concentrations of 0.87 mu M and 1.74 mu M. In addition, multiparametric cytotoxicity assays were performed in the human hepatoma HepaRG cell line, which showed that C17-SAMT induced mitochondrial dysfunction, decreased cellular ATP levels, and altered the expression of various proteins, including superoxide dismutase SOD2, heme oxygenase HO-1, and NF-kappa B. These results suggest that C17-SAMT is mutagenic in vitro and can induce mitochondrial dysfunction in HepaRG cells. However, the exact mode of action of this toxin requires further investigation. Overall, this study highlights the potential toxicity of C17-SAMT and the need for further research to better understand its effects.
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