Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/ijms241210228
Keywords
Toxoplasma gondii; TgMORN2; endoplasmic reticulum stress
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MORN proteins play a crucial role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. Through adjacent protein labeling techniques, we identified a network of interactions involving TgMORN2 and endoplasmic reticulum stress-related proteins. The pathogenicity of the KO-TgMORN2 strain was significantly reduced under tunicamycin-induced ER stress. Overall, TgMORN2 functions in ER stress and provides a basis for further research on the function of MORN proteins in Toxoplasma gondii.
MORN proteins play a key role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. A gene with nine MORN motifs (TGGT1_292120, named TgMORN2) was identified in the Toxoplasma gondii genome; it was presumed to belong to the MORN protein family and to have the function of forming the cytoskeleton, which affects the survival of T. gondii. However, the genetic deletion of MORN2 did not noticeably affect parasite growth and virulence. Using adjacent protein labeling techniques, we identified a network of TgMORN2 interactions, which mainly included endoplasmic reticulum stress (ER stress)-related proteins. In exploring these data, we found that the pathogenicity of the KO-TgMORN2 strain was significantly reduced in the case of tunicamycin-induced ER stress. Reticulon TgRTN (TGGT1_226430) and tubulin & beta;-Tubulin were identified as interaction proteins of TgMORN2. Collectively, TgMORN2 plays a role in ER stress, which lays a foundation for further research on the function of the MORN protein in T. gondii.
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