Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms24076073
Keywords
carvacrol; p-cymene; Alzheimer's disease; diabetes mellitus; natural compounds
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Several researchers have studied the health properties of carvacrol and p-cymene, substances found in aromatic plants. In this study, the researchers investigated the potential anti-cholinesterase, anti-alpha-amylase, and neuroprotective effects of these compounds. They found that carvacrol showed inhibitory activities against acetylcholinesterase and butyrylcholinesterase, while also exhibiting anti-alpha-amylase activity. Furthermore, pre-treatment with carvacrol and p-cymene reduced caspase-3 expression in cells under oxidative stress, indicating their potential as neuroprotective agents.
Several researchers have demonstrated the health and pharmacological properties of carvacrol and p-cymene, monoterpenes of aromatic plants. This study investigated these compounds' possible anti-cholinesterase, anti-alpha-amylase, and neuroprotective effects. We evaluated the anti-acetylcholinesterase and anti-alpha-amylase activities at different concentrations of the compounds. The maximum non-toxic dose of carvacrol and p-cymene against SH-SY5Y neuroblastoma cells was determined using an MTT assay. The neuroprotective effects of the compounds were evaluated on H2O2-induced stress in SH-SY5Y cells, studying the expression of caspase-3 usingWestern blotting assays. Carvacrol showed inhibitory activities against acetylcholinesterase (IC50 = 3.8 mu g/mL) and butyrylcholinesterase (IC50 = 32.7 mu g/mL). Instead, the anti-alpha-amylase activity of carvacrol resulted in an IC50 value of 171.2 mu g/mL After a pre-treatment with the maximum non-toxic dose of carvacrol and p-cymene, the expression of caspase-3 was reduced compared to cells treated with H2O2 alone. Carvacrol and p-cymene showed in vitro anti-enzymatic properties, and may act as neuroprotective agents against oxidative stress. Further studies are necessary to elucidate their possible use as coadjutants in preventing and treating AD in diabetic patients.
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