Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/ijms241310473
Keywords
cervical cancer; Parallel Reaction Monitoring (PRM); minichromosome maintenance-3 (MCM3); ThinPrep; protein biomarker; early diagnosis
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By using a targeted proteomics approach, it was found that the concentration levels of MCM3 and EVPL proteins can discriminate between non-dysplastic and dysplastic samples, improving the accuracy of cervical cancer screening. This suggests that MCM3 and EVPL may be promising candidate protein biomarkers for population-based cervical cancer screening.
Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital's outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with 'negative for intraepithelial lesion or malignancy' (NILM), 21 samples with 'atypical squamous cells of undetermined significance' (ASC-US), and 33 samples with 'low-grade squamous intraepithelial lesion and worse' (& GE;LSIL) were analyzed, using cytology and the patients' histology reports. Highly accurate concordance was obtained for gold-standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.
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